Show Order Info
SKU:PB9013
Clonality:Polyclonal
Application:WB
Price: $240.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-IL-2 Antibody
Description Rabbit IgG polyclonal antibody for Interleukin-2(IL-2) detection. Tested with WB in Rat.
Cite This Product Anti-IL-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9013)
Replacement Item This antibody may replace the following items: sc-1103|sc-1104|sc-133118|sc-137119|sc-1697|sc-23902|sc-32295|sc-34796|sc-393947|sc-398253|sc-4593|sc-48544|sc-48545|sc-4879|sc-514176|sc-52017|sc-56383|sc-65357|sc-71040|sc-71041|sc-7896 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived rat IL-2 recombinant protein (Position: A21-Q155). Rat IL-2 shares 66% and 80% amino acid (aa) sequences identity with human and mouse IL-2, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Il2
Protein Name Interleukin-2
Molecular Weight 17633 MW
Protein Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.
Subcellular Localization Secreted.
Uniprot ID P17108
Alternative Names Interleukin-2;IL-2;T-cell growth factor;TCGF;Il2;Il-2;
Research Areas |immunology|adaptive immunity|t cells| immunology|cell type markers|cytokines|innate immunity|interleukins|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Interleukin-2

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. This gene for interleukin 2 (IL2) is assigned to chromosome 4. IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Anti-IL-2 Antibody Images

Click the images to enlarge.

Anti-IL-2 Antibody
Anti-IL-2 Picoband antibody, PB9013-1.jpg
All lanes: Anti-IL-2(PB9013) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Liver Tissue Lysate at 40ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: PC12 Whole Cell Lysate at 40ug
Lane 5: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
Write a review for PB9013

Publications

Zhong B, Shen H, Sun X, Wang H, Zhang Y, Sun Z. Cancer Biother Radiopharm. 2012 May;27(4):252-8. Doi: 10.1089/Cbr.2011.1105. Additive Effects Of Ulinastatin And Docetaxel On Growth Of Breast Cancer Xenograft In Nude Mice And Expression Of Pge2, Il...
Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.
Xie Ft, Cao Js, Zhao J, Yu Y, Qi F, Dai Xc. Transpl Immunol. 2015 Oct;33(2):69-77. Doi: 10.1016/J.Trim.2015.05.003. Epub 2015 May 20. Ido Expressing Dendritic Cells Suppress Allograft Rejection Of Small Bowel Transplantation In Mice By Expansion O...
Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to il 2 antibody, il-2 antibody, il2 antibody, interleukin 2 antibody, interleukin-2 antibody