Anti-IL-31 Antibody Picoband™

IL-31 antibody

Boster Bio Anti-IL-31 Antibody Picoband™ catalog # A08339-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A08339-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-IL-31 Antibody Picoband™

View all IL-31 Antibodies

SKU/Catalog Number

A08339-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL-31 Antibody Picoband™ catalog # A08339-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL-31 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08339-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human IL-31 recombinant protein (Position: S24-T164).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08339-1 is reactive to Il31 in Human, Mouse, Rat

Applications

A08339-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

18.12kDa

Background of IL-31

Interleukin-31 (IL-31) is a protein that in humans is encoded by the IL31 gene that resides on chromosome 12. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Il31 (Source: Uniprot.org, NCBI)

Gene Name

Il31

Full Name

Interleukin-31

Weight

18.12kDa

Alternative Names

IL31; IL-31; IL-31interleukin-31; interleukin 31 Il31|1700013B14Rik|interleukin 31|interleukin-31|IL-31

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Il31, check out the Il31 Infographic

Il31 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Il31: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-IL-31 Antibody Picoband™

Question

Will A08339-1 anti-IL-31 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-15

Answer

You can see on the product datasheet, A08339-1 anti-IL-31 antibody as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-15

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-IL-31 antibody A08339-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-02

Question

Is this A08339-1 anti-IL-31 antibody reactive to the isotypes of IL31?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

The immunogen of A08339-1 anti-IL-31 antibody is E.coli-derived human IL-31 recombinant protein (Position: S24-T164). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-05

Question

Do you have a BSA free version of anti-IL-31 antibody A08339-1 available?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL-31 antibody A08339-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-22

Question

My colleagues were happy with the WB result of your anti-IL-31 antibody. However we have been able to see positive staining in testis secreted. using this antibody. Is that expected? Could you tell me where is IL31 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

From literature, testis does express IL31. Generally IL31 expresses in secreted. Regarding which tissues have IL31 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 15184896

Boster Scientific Support

Answered: 2019-11-15

Question

Does anti-IL-31 antibody A08339-1 work for IHC-P with peripheral blood?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

According to the expression profile of peripheral blood, IL31 is highly expressed in peripheral blood. So, it is likely that anti-IL-31 antibody A08339-1 will work for IHC-P with peripheral blood.

Boster Scientific Support

Answered: 2019-10-04

Question

I am interested in to test anti-IL-31 antibody A08339-1 on mouse peripheral blood for research purposes, then I may be interested in using anti-IL-31 antibody A08339-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

The products we sell, including anti-IL-31 antibody A08339-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-23

Question

See below the WB image, lot number and protocol we used for peripheral blood using anti-IL-31 antibody A08339-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-19

Question

I was wanting to use your anti-IL-31 antibody for IHC-P for mouse peripheral blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse peripheral blood identification?

Verified Customer

Verified customer

Asked: 2019-03-04

Answer

It shows on the product datasheet, A08339-1 anti-IL-31 antibody has been validated for ELISA, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse peripheral blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-04

Question

We have tried in the past anti-IL-31 antibody for WB on peripheral blood in the past. I am using mouse, and I plan to use the antibody for IHC-P next. My lab would like examining peripheral blood as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2018-03-02

Answer

I took a look at the website and datasheets of our anti-IL-31 antibody and it appears that A08339-1 has been validated on mouse in both WB and IHC-P. Thus A08339-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-03-02

Question

Is a blocking peptide available for product anti-IL-31 antibody (A08339-1)?

J. Edwards

Verified customer

Asked: 2017-10-26

Answer

We do provide the blocking peptide for product anti-IL-31 antibody (A08339-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-26

Question

I see that the anti-IL-31 antibody A08339-1 works with IHC-P, what is the protocol used to produce the result images on the product page?

R. Parker

Verified customer

Asked: 2017-06-27

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-27

Question

We are currently using anti-IL-31 antibody A08339-1 for human tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-16

Answer

The anti-IL-31 antibody (A08339-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-16

Question

Can you help my question with product A08339-1, anti-IL-31 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Taylor

Verified customer

Asked: 2016-12-20

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08339-1 anti-IL-31 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-12-20

Question

We have been able to see staining in human testis. What should we do? Is anti-IL-31 antibody supposed to stain testis positively?

T. Singh

Verified customer

Asked: 2016-02-08

Answer

From what I have seen in literature testis does express IL31. From what I have seen in Uniprot.org, IL31 is expressed in testis, peripheral blood, among other tissues. Regarding which tissues have IL31 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 15184896

Boster Scientific Support

Answered: 2016-02-08

Question

I would like using your anti-IL-31 antibody for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.

A. Gonzalez

Verified customer

Asked: 2015-09-29

Answer

Thank you for your inquiry. This A08339-1 anti-IL-31 antibody is tested on rat thymus tissue, spleen tissue. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-09-29

Order DetailsPrice
A08339-1

100μg

$370
A08339-1-10ug

10μg sample (liquid)

$99
A08339-1-Biotin

100 μg Biotin conjugated

$570
A08339-1-Cy3

100 μg Cy3 conjugated

$570
A08339-1-Dylight488

100 μg Dylight488 conjugated

$570
A08339-1-Dylight550

100 μg Dylight550 conjugated

$570
A08339-1-Dylight594

100 μg Dylight594 conjugated

$570
A08339-1-FITC

100 μg FITC conjugated

$570
A08339-1-HRP

100 μg HRP conjugated

$570
A08339-1-APC

100 μg APC conjugated

$670
A08339-1-PE

100 μg PE conjugated

$670
A08339-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A08339-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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