Anti-Interleukin-6 IL6 Antibody

IL-6 antibody

Boster Bio Anti-Interleukin-6 IL6 Antibody catalog # PA1352. Tested in WB applications. This antibody reacts with Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1352
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Interleukin-6 IL6 Antibody

View all IL-6 Antibodies

SKU/Catalog Number

PA1352

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-6 IL6 Antibody catalog # PA1352. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-6 IL6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1352)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1352 is reactive to IL6 in Mouse, Rat

Applications

PA1352 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

23.718kDa

Background of IL-6

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL6 gene to chromosome 7p15.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For IL6 (Source: Uniprot.org, NCBI)

Gene Name

IL6

Full Name

Interleukin-6

Weight

23.718kDa

Superfamily

IL-6 superfamily

Alternative Names

B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL6, check out the IL6 Infographic

IL6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1352 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Tan J,Luo J,Meng C,Jiang N,Cao J,Zhao J. Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway. Int Immunopharmacol.2020 Dec 11;90:107268.doi:10.1016/j. intimp.2020.107268.Epub ahead of print.PMID:333
Species: Rat
PA1352 usage in article: APP:WB, SAMPLE:CEREBRAL CORTEX, DILUTION:NA

Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-%u03B21/Smad Pathways

Transfection with CXCR4 potentiates homing of mesenchymal stem cells in vitro and therapy of diabetic retinopathy in vivo

The expression and significance of IL-6, IFN-%u03B3, SM22%u03B1, and MMP-2 in rat model of aortic dissection

Distribution change of mast cells in human nasal polyps

The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway

Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm

Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats

Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells

Have you used Anti-Interleukin-6 IL6 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Interleukin-6 IL6 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Interleukin-6 IL6 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL6 antibody PA1352. Let me know if you need anything else.

A. Moore

Verified customer

Asked: 2020-04-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-16

Question

Is this PA1352 anti-IL6 antibody reactive to the isotypes of IL6?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

The immunogen of PA1352 anti-IL6 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6(195-211aa KALEEFLKVTMRSTRQT), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-27

Question

Do you have a BSA free version of anti-IL6 antibody PA1352 available?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL6 antibody PA1352 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-24

Question

Will anti-IL6 antibody PA1352 work on zebrafish WB with left coronary artery?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

Our lab technicians have not validated anti-IL6 antibody PA1352 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL6 antibody PA1352 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish left coronary artery in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-14

Question

I was wanting to use your anti-IL6 antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

It shows on the product datasheet, PA1352 anti-IL6 antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-15

Question

Is a blocking peptide available for product anti-IL6 antibody (PA1352)?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

We do provide the blocking peptide for product anti-IL6 antibody (PA1352). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-16

Question

We are currently using anti-IL6 antibody PA1352 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on goat tissues as well?

L. Gonzalez

Verified customer

Asked: 2019-09-03

Answer

The anti-IL6 antibody (PA1352) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-03

Question

We need to test anti-IL6 antibody PA1352 on mouse lung for research purposes, then I may be interested in using anti-IL6 antibody PA1352 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

The products we sell, including anti-IL6 antibody PA1352, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-06

Question

See below the WB image, lot number and protocol we used for lung using anti-IL6 antibody PA1352. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-07

Question

I see that the anti-IL6 antibody PA1352 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-03-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-03-08

Question

Does anti-IL6 antibody PA1352 work for WB with lung?

Verified Customer

Verified customer

Asked: 2018-12-03

Answer

According to the expression profile of lung, IL6 is highly expressed in lung. So, it is likely that anti-IL6 antibody PA1352 will work for WB with lung.

Boster Scientific Support

Answered: 2018-12-03

Question

I have a question about product PA1352, anti-IL6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1352 anti-IL6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-03

Question

We have seen staining in mouse left coronary artery. Are there any suggestions? Is anti-IL6 antibody supposed to stain left coronary artery positively?

G. Mangal

Verified customer

Asked: 2015-07-30

Answer

From literature left coronary artery does express IL6. From Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-07-30

Question

Our team were satisfied with the WB result of your anti-IL6 antibody. However we have been able to see positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?

L. Jones

Verified customer

Asked: 2014-07-09

Answer

From what I have seen in literature, left coronary artery does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-07-09

Question

Would PA1352 anti-IL6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Carter

Verified customer

Asked: 2013-01-31

Answer

It shows on the product datasheet, PA1352 anti-IL6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-31

Order DetailsPrice
PA1352

100μg

$370
PA1352-10ug

10μg sample (liquid)

$99
PA1352-Biotin

100 μg Biotin conjugated

$570
PA1352-Cy3

100 μg Cy3 conjugated

$570
PA1352-Dylight488

100 μg Dylight488 conjugated

$570
PA1352-Dylight550

100 μg Dylight550 conjugated

$570
PA1352-Dylight594

100 μg Dylight594 conjugated

$570
PA1352-FITC

100 μg FITC conjugated

$570
PA1352-HRP

100 μg HRP conjugated

$570
PA1352-APC

100 μg APC conjugated

$670
PA1352-PE

100 μg PE conjugated

$670
PA1352-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PA1352
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.