Anti-IL6 Antibody Picoband™

IL-6 antibody

Boster Bio Anti-IL6 Antibody Picoband™ catalog # PB9005. Tested in WB applications. This antibody reacts with Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9005
Size: 100 μg/vial
Reactive Species: Rat
Host: Rabbit
Application: WB

Product Name

Anti-IL6 Antibody Picoband™

View all IL-6 Antibodies

SKU/Catalog Number

PB9005

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL6 Antibody Picoband™ catalog # PB9005. Tested in WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9005)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat IL-6 recombinant protein (Position: F25-T211). Rat IL-6 shares 86% amino acid (aa) sequence identity with mouse IL-6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9005 is reactive to IL6 in Rat

Applications

PB9005 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

23.718kDa

Background of IL-6

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL-6 gene to chromosome 7p15.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For IL6 (Source: Uniprot.org, NCBI)

Gene Name

IL6

Full Name

Interleukin-6

Weight

23.718kDa

Superfamily

IL-6 superfamily

Alternative Names

B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL6, check out the IL6 Infographic

IL6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9005 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Tan J,Luo J,Meng C,Jiang N,Cao J,Zhao J. Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway. Int Immunopharmacol.2020 Dec 11;90:107268.doi:10.1016/j. intimp.2020.107268.Epub ahead of print.PMID:333
Species: Rat
PB9005 usage in article: APP:WB, SAMPLE:CEREBRAL CORTEX, DILUTION:NA

Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-%u03B21/Smad Pathways

Transfection with CXCR4 potentiates homing of mesenchymal stem cells in vitro and therapy of diabetic retinopathy in vivo

The expression and significance of IL-6, IFN-%u03B3, SM22%u03B1, and MMP-2 in rat model of aortic dissection

Distribution change of mast cells in human nasal polyps

The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway

Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm

Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats

Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells

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3 Customer Q&As for Anti-IL6 Antibody Picoband™

Question

We have observed staining in rat fibroblast. What should we do? Is anti-IL6 antibody supposed to stain fibroblast positively?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

According to literature fibroblast does express IL6. According to Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-03

Question

My team were happy with the WB result of your anti-IL6 antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?

B. Miller

Verified customer

Asked: 2015-06-10

Answer

Based on literature, lung does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2015-06-10

Question

We are currently using anti-IL6 antibody PB9005 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on monkey tissues as well?

J. Miller

Verified customer

Asked: 2013-08-06

Answer

The anti-IL6 antibody (PB9005) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-08-06

Order DetailsPrice
PB9005

100μg

$370
PB9005-10ug

10μg sample (liquid)

$99
PB9005-Biotin

100 μg Biotin conjugated

$570
PB9005-Cy3

100 μg Cy3 conjugated

$570
PB9005-Dylight488

100 μg Dylight488 conjugated

$570
PB9005-Dylight550

100 μg Dylight550 conjugated

$570
PB9005-Dylight594

100 μg Dylight594 conjugated

$570
PB9005-FITC

100 μg FITC conjugated

$570
PB9005-HRP

100 μg HRP conjugated

$570
PB9005-APC

100 μg APC conjugated

$670
PB9005-PE

100 μg PE conjugated

$670
PB9005-carrier-free

Carrier Free

$370

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