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SKU:PB9034
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:ELISA, WB
Price: $240.00
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Overview

Product Name Anti-IL-6 Antibody
SKU/Catalog Number PB9034
Description Rabbit IgG polyclonal antibody for Interleukin-6(IL6) detection. Tested with WB, ELISA in Mouse.
Cite This Product Anti-IL-6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9034)
Replacement Item This antibody may replace the following items: sc-130326|sc-13087|sc-1356|sc-1357|sc-135733|sc-135733-X|sc-135734|sc-135734-X|sc-1358|sc-150|sc-150-G|sc-150-X|sc-151|sc-151-X|sc-166724|sc-28343|sc-32296|sc-323975|sc-323976|sc-34461|sc-34462|sc-365546|sc-374422|sc-398753|sc-398753-X|sc-515028|sc-515028-X|sc-53301|sc-53478|sc-53865|sc-56637|sc-57193|sc-636|sc-636-X|sc-65327|sc-65989|sc-71244|sc-746|sc-746-X|sc-7920|sc-7949|sc-7962|sc-7962-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse
Application ELISA, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived mouse IL-6 recombinant protein (Position: F25-T211). Mouse IL-6 shares 40% and 85% amino acid (aa) sequences identity with human and rat IL-6, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Il6
Protein Name Interleukin-6
Molecular Weight 24384 MW
Protein Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. .
Subcellular Localization Secreted.
Uniprot ID P08505
Alternative Names Interleukin-6;IL-6;B-cell hybridoma growth factor;Interleukin HP-1;Il6;Il-6;
Research Areas |immunology|innate immunity|cytokines|interleukins| neuroscience|neurology process|metabolism| cancer|invasion/microenvironment|angiogenesis|angiogenic growth factors|tumor immunology| metabolism|types of disease|obesity|metabolic disorders|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Interleukin-6

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL-6 gene to chromosome 7p15.

Anti-IL-6 Antibody Images

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Anti-IL-6 Antibody
Anti-IL-6 Picoband antibody, PB9034-1.jpg
All lanes: Anti-IL-6(PB9034) at 0.5ug/ml
Lane 1: HEPA Whole Cell Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
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Publications

Liao Q, Zeng Z, Guo X, Li X, Wei F, Zhang W, Li X, Chen P, Liang F, Xiang B, Ma J, Wu M, Tang H, Deng M, Zeng X, Tang K, Xiong W, Li G. Oncogene. 2014 Apr 17;33(16):2098-109. Doi: 10.1038/Onc.2013.161. Epub 2013 May 27. Lplunc1 Suppresses Il-6-Ind...
Cui L, Feng L, Zhang Zh, Jia Xb. Int Immunopharmacol. 2014 Nov;23(1):294-303. Doi: 10.1016/J.Intimp.2014.09.005. Epub 2014 Sep 18. The Anti-Inflammation Effect Of Baicalin On Experimental Colitis Through Inhibiting Tlr4/Nf-??b Pathway Activation.
Ye Q, Zheng Y, Fan S, Qin Z, Li N, Tang A, Ai F, Zhang X, Bian Y, Dang W, Huang J, Zhou M, Zhou Y, Xiong W, Yan Q, Ma J, Li G. Plos One. 2014 Jul 24;9(7):E103298. Doi: 10.1371/Journal.Pone.0103298. Ecollection 2014. Lactoferrin Deficiency Promotes...
Zhao H, Li X, Li N, Liu T, Liu J, Li Z, Xiao H, Li J. Br J Nutr. 2014 Mar 14;111(5):836-46. Doi: 10.1017/S0007114513003115. Epub 2013 Sep 30. Long-Term Resveratrol Treatment Prevents Ovariectomy-Induced Osteopenia In Rats Without Hyperplastic Effe...
Li J, Zhang J, Fu Y, Sun X, Gong T, Jiang J, Zhang Z. J Control Release. 2015 Aug 28;212:19-29. Doi: 10.1016/J.Jconrel.2015.06.011. Epub 2015 Jun 11. Dual Pancreas- And Lung-Targeting Therapy For Local And Systemic Complications Of Acute Pancreati...
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Huang Q, Du J, Fan J, Lv Z, Qian X, Zhang X, Han J, Chen C, Wu F, Jin Y. Med Oncol. 2014 Sep;31(9):144. Doi: 10.1007/S12032-014-0144-Z. Epub 2014 Aug 12. The Effect Of Proinflammatory Cytokines On Il-17Ra Expression In Nsclc.
Liu T, Zeng Z, Liu Y, Wang J, Maitz Mf, Wang Y, Liu S, Chen J, Huang N. Acs Appl Mater Interfaces. 2014 Jun 11;6(11):8729-43. Doi: 10.1021/Am5015309. Epub 2014 Apr 23. Surface Modification With Dopamine And Heparin/Poly-L-Lysine Nanoparticles Prov...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Beari...
Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y. Plos One. 2014 Jun 30;9(6):E101291. Doi: 10.1371/Journal.Pone.0101291. Ecollection 2014. Ginsenoside Rg1 Prevents Cognitive Impairment And Hippocampus Senescence In A Rat Model...
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induced...
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to il 6 antibody, il-6 antibody, il6 antibody, interleukin 6 antibody, interleukin-6 antibody