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Product Info Summary
| SKU: | A00423-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC-P |
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Product info
Product Name
Anti-IL-8 CXCL8 Monoclonal Antibody
View all CXCL8/IL-8 Antibodies
SKU/Catalog Number
A00423-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-8 CXCL8 Monoclonal Antibody catalog # A00423-1. Tested in IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-8 CXCL8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00423-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
8B1
Isotype
IgG1
Immunogen
synthetic peptide derived from IL-8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00423-1 is reactive to CXCL8 in Human, Mouse, Rat
Applications
A00423-1 is guaranteed for IHC-P Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
IHC, 1:100-200
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Carcinoma Tissue using IL-8 Mouse Monoclonal antibody diluted at 1:200.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Kidney Tissue using IL-8 Mouse Monoclonal antibody diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For CXCL8 (Source: Uniprot.Org, NCBI)
Uniprot ID
P10145
Gene ID
3576
Gene Name
CXCL8
Full Name
Interleukin-8
Weight
11.098kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 CXCL8 GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8 C-X-C motif chemokine ligand 8 interleukin-8|T-cell chemotactic factor|alveolar macrophage chemotactic factor I|beta endothelial cell-derived neutrophil activating peptide|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic protein 1|interleukin 8|lung giant cell carcinoma-derived chemotactic protein|lymphocyte derived neutrophil activating peptide|monocyte-derived neutrophil chemotactic factor|monocyte-derived neutrophil-activating peptide|neutrophil-activating peptide 1|small inducible cytokine subfamily B, member 8|tumor necrosis factor-induced gene 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL8, check out the CXCL8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-IL-8 CXCL8 Monoclonal Antibody (A00423-1)
A00423-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus
Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögrens Syndrome-Associated Thrombocytopenia
Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
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15 Customer Q&As for Anti-IL-8 CXCL8 Monoclonal Antibody
Question
I was wanting to use your anti-IL-8 Monoclonal antibody for IHC for rat neutrophil on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat neutrophil identification?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
It shows on the product datasheet, A00423-1 anti-IL-8 Monoclonal antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat neutrophil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neutrophil using anti-IL-8 Monoclonal antibody A00423-1. Let me know if you need anything else.
R. Bhatt
Verified customer
Asked: 2020-04-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
See below the WB image, lot number and protocol we used for neutrophil using anti-IL-8 Monoclonal antibody A00423-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
My colleagues were content with the WB result of your anti-IL-8 Monoclonal antibody. However we have been able to see positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is CXCL8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
According to literature, lung does express CXCL8. Generally CXCL8 expresses in secreted. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384
Boster Scientific Support
Answered: 2020-02-24
Question
I am interested in using your anti-IL-8 Monoclonal antibody for neutrophil activation studies. Has this antibody been tested with western blotting on human kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
We appreciate your inquiry. This A00423-1 anti-IL-8 Monoclonal antibody is validated on human kidney tissue, lung carcinoma tissue. It is guaranteed to work for IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-06
Question
Is this A00423-1 anti-IL-8 Monoclonal antibody reactive to the isotypes of CXCL8?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The immunogen of A00423-1 anti-IL-8 Monoclonal antibody is synthetic peptide derived from IL-8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-16
Question
Is there a BSA free version of anti-IL-8 Monoclonal antibody A00423-1 available?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-8 Monoclonal antibody A00423-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-21
Question
We need to test anti-IL-8 Monoclonal antibody A00423-1 on rat neutrophil for research purposes, then I may be interested in using anti-IL-8 Monoclonal antibody A00423-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The products we sell, including anti-IL-8 Monoclonal antibody A00423-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-IL-8 Monoclonal antibody A00423-1 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-IL-8 Monoclonal antibody (A00423-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
I see that the anti-IL-8 Monoclonal antibody A00423-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-25
Question
Would A00423-1 anti-IL-8 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Jones
Verified customer
Asked: 2018-07-12
Answer
As indicated on the product datasheet, A00423-1 anti-IL-8 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-12
Question
Will anti-IL-8 Monoclonal antibody A00423-1 work for IHC with neutrophil?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
According to the expression profile of neutrophil, CXCL8 is highly expressed in neutrophil. So, it is likely that anti-IL-8 Monoclonal antibody A00423-1 will work for IHC with neutrophil.
Boster Scientific Support
Answered: 2018-06-05
Question
We have been able to see staining in rat skin. Are there any suggestions? Is anti-IL-8 Monoclonal antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
According to literature skin does express CXCL8. According to Uniprot.org, CXCL8 is expressed in periodontal ligament, lung carcinoma, neutrophil, lung, peripheral blood leukocyte, kidney, skin, chronic myeloid leukemia cell, among other tissues. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384
Boster Scientific Support
Answered: 2017-12-13
Question
I have a question about product A00423-1, anti-IL-8 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Miller
Verified customer
Asked: 2017-05-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00423-1 anti-IL-8 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-17
Question
Is a blocking peptide available for product anti-IL-8 Monoclonal antibody (A00423-1)?
R. Mitchell
Verified customer
Asked: 2014-08-15
Answer
We do provide the blocking peptide for product anti-IL-8 Monoclonal antibody (A00423-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-08-15
