Overview
| Product Name |
Anti-IL-8 CXCL8 Monoclonal Antibody
See more CXCL8/IL-8 products |
| Catalog# |
A00423-1 |
| Pack Size |
100μl |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-IL-8 CXCL8 Monoclonal Antibody catalog # A00423-1. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat. |
| Cite This Product |
Anti-IL-8 CXCL8 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00423-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-IL-8 CXCL8 Monoclonal Antibody may replace the following items: sc 25342|sc 55462|sc 7165|sc 4600|sc 73321|sc 7922|sc 1269|sc 376750|sc 8427|sc 7302|sc 52870|sc 101499|sc 32817|sc 71367. |
Product Specs
| Host |
Mouse |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IHC
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
synthetic peptide derived from IL-8 |
Gene/Protein Basic Information For CXCL8 (Source: Uniprot.org, NCBI)
| Uniprot Id | P10145 |
|---|
| NCBI Gene Id | 3576 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | CXCL8 |
|---|
| Protein Name | Interleukin-8 |
|---|
| Superfamily | intercrine alpha (chemokine CxC) family |
|---|
| Alternative Names | CXCL8/IL-8|3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 |
|---|
| Molecular Weight | 11098 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CXCL8, check out the CXCL8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CXCL8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the CXCL8 infographic
Our Boster Quality Guarantee for Anti-IL-8 CXCL8 Monoclonal Antibody covers its use in the following applications.
IHC, 1:100-200
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Carcinoma Tissue using IL-8 Mouse Monoclonal antibody diluted at 1:200.
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Kidney Tissue using IL-8 Mouse Monoclonal antibody diluted at 1:200.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus |
PubMed ID 25317156 |
|
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Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to A00423-1 Anti-IL-8 CXCL8 Monoclonal Antibody
15 Related Questions
Question
I was wanting to use your anti-IL-8 Monoclonal antibody for IHC for rat neutrophil on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat neutrophil identification?
Verified Customer
Asked: 2020-05-06
Answer
It shows on the product datasheet, A00423-1 anti-IL-8 Monoclonal antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat neutrophil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neutrophil using anti-IL-8 Monoclonal antibody A00423-1. Let me know if you need anything else.
R. Bhatt
Asked: 2020-04-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
See below the WB image, lot number and protocol we used for neutrophil using anti-IL-8 Monoclonal antibody A00423-1. Please let me know if you require anything else.
Verified Customer
Asked: 2020-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
My colleagues were content with the WB result of your anti-IL-8 Monoclonal antibody. However we have been able to see positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is CXCL8 supposed to be expressed?
Verified Customer
Asked: 2020-02-24
Answer
According to literature, lung does express CXCL8. Generally CXCL8 expresses in secreted. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384
Boster Scientific Support
Answered: 2020-02-24
Question
I am interested in using your anti-IL-8 Monoclonal antibody for neutrophil activation studies. Has this antibody been tested with western blotting on human kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Asked: 2020-01-06
Answer
We appreciate your inquiry. This A00423-1 anti-IL-8 Monoclonal antibody is validated on human kidney tissue, lung carcinoma tissue. It is guaranteed to work for IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-06
Question
Is this A00423-1 anti-IL-8 Monoclonal antibody reactive to the isotypes of CXCL8?
Verified Customer
Asked: 2019-12-16
Answer
The immunogen of A00423-1 anti-IL-8 Monoclonal antibody is synthetic peptide derived from IL-8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-16
Question
Is there a BSA free version of anti-IL-8 Monoclonal antibody A00423-1 available?
Verified Customer
Asked: 2019-11-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-8 Monoclonal antibody A00423-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-21
Question
We need to test anti-IL-8 Monoclonal antibody A00423-1 on rat neutrophil for research purposes, then I may be interested in using anti-IL-8 Monoclonal antibody A00423-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-08-26
Answer
The products we sell, including anti-IL-8 Monoclonal antibody A00423-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-IL-8 Monoclonal antibody A00423-1 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Asked: 2019-05-31
Answer
The anti-IL-8 Monoclonal antibody (A00423-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
I see that the anti-IL-8 Monoclonal antibody A00423-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2018-07-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-25
Question
Would A00423-1 anti-IL-8 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Jones
Asked: 2018-07-12
Answer
As indicated on the product datasheet, A00423-1 anti-IL-8 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-12
Question
Will anti-IL-8 Monoclonal antibody A00423-1 work for IHC with neutrophil?
Verified Customer
Asked: 2018-06-05
Answer
According to the expression profile of neutrophil, CXCL8 is highly expressed in neutrophil. So, it is likely that anti-IL-8 Monoclonal antibody A00423-1 will work for IHC with neutrophil.
Boster Scientific Support
Answered: 2018-06-05
Question
We have been able to see staining in rat skin. Are there any suggestions? Is anti-IL-8 Monoclonal antibody supposed to stain skin positively?
Verified Customer
Asked: 2017-12-13
Answer
According to literature skin does express CXCL8. According to Uniprot.org, CXCL8 is expressed in periodontal ligament, lung carcinoma, neutrophil, lung, peripheral blood leukocyte, kidney, skin, chronic myeloid leukemia cell, among other tissues. Regarding which tissues have CXCL8 expression, here are a few articles citing expression in various tissues:
Chronic myeloid leukemia cell, Pubmed ID: 8344717
Kidney, Pubmed ID: 2523801
Lung, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 2200751
Neutrophil, Pubmed ID: 14702039
Skin, Pubmed ID: 1755384
Boster Scientific Support
Answered: 2017-12-13
Question
I have a question about product A00423-1, anti-IL-8 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Miller
Asked: 2017-05-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00423-1 anti-IL-8 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-17
Question
Is a blocking peptide available for product anti-IL-8 Monoclonal antibody (A00423-1)?
R. Mitchell
Asked: 2014-08-15
Answer
We do provide the blocking peptide for product anti-IL-8 Monoclonal antibody (A00423-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-08-15
