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SKU:PA1353
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-IL-8 Antibody
Description Rabbit IgG polyclonal antibody for Interleukin-8(CXCL8) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-IL-8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1353)
Replacement Item This antibody may replace the following items: sc-101499|sc-1269|sc-32817|sc-376750|sc-4600|sc-52870|sc-7302|sc-73321|sc-7922|sc-8427 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human IL8(77-95aa CLDPKENWVQRVVEKFLKR).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CXCL8
Protein Name Interleukin-8(IL-8)
Molecular Weight 11098 MW
Protein Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. .
Sequence Similarities Belongs to the intercrine alpha (chemokine CxC) family.
Subcellular Localization Secreted.
Uniprot ID P10145
Alternative Names Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8;(Ala-IL-8)77;GCP/IL-8 protein II;IL-8(1-77);IL8/NAP1 form II;MDNCF-b;IL-8(5-77);IL-8(6-77);(Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8(7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8(8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8(9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8;
Research Areas |cardiovascular|angiogenesis|cytokines|interleukin| immunology|innate immunity|interleukins| cancer|invasion/microenvironment|angiogenic growth factors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Interleukin-8(IL-8)

Interleukin-8(IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8(IL8) is a member of the CXC chemokine family(Hull et al., 2001). IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.

Anti-IL-8 Antibody Images

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Anti-IL-8 Antibody
Anti-IL-8 antibody, PA1353, Western blotting
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Anti-IL-8 Antibody
Anti-IL-8 antibody, PA1353, IHC(P)
IHC(P): Human Appendicitis Tissue
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Publications

Wang B, Li K, Jin W, Lu Y, Zhang Y, Shen G, Wang R, Shen H, Li W, Huang Y, Zhang Y, Wang X, Li X, Liu W, Cao H, Tao S. Environ Sci Technol. 2013 Sep 17;47(18):10583-90. Doi: 10.1021/Es401394G. Epub 2013 Sep 4. Properties And Inflammatory Effects O...
Huang Q, Du J, Fan J, Lv Z, Qian X, Zhang X, Han J, Chen C, Wu F, Jin Y. Med Oncol. 2014 Sep;31(9):144. Doi: 10.1007/S12032-014-0144-Z. Epub 2014 Aug 12. The Effect Of Proinflammatory Cytokines On Il-17Ra Expression In Nsclc.
Li J, Yang L, Yao Q, Li Y, Tian Y, Li S, Jiang S, Wang Y, Li X, Guo Z. Evid Based Complement Alternat Med. 2014;2014:381976. Doi: 10.1155/2014/381976. Epub 2014 May 14. Effects And Mechanism Of Bufei Yishen Formula In A Rat Chronic Obstructive Pul...
Dai Jp, Zhao Xf, Zeng J, Wan Qy, Yang Jc, Li Wz, Chen Xx, Wang Gf, Li Ks. Plos One. 2013 Apr 16;8(4):E61026. Doi: 10.1371/Journal.Pone.0061026. Print 2013. Drug Screening For Autophagy Inhibitors Based On The Dissociation Of Beclin1-Bcl2 Complex U...
Xie Xj, Ye Yf, Zhou L, Xie Hy, Jiang Gp, Feng Xw, He Y, Xie Qf, Zheng Ss. J Zhejiang Univ Sci B. 2010 Nov;11(11):819-27. Doi: 10.1631/Jzus.B1000030. Th17 Promotes Acute Rejection Following Liver Transplantation In Rats.
Dong Sj, Zhong Yq, Lu Wt, Li Gh, Jiang Hl, Mao B. Inflammation. 2015 Aug;38(4):1493-501. Doi: 10.1007/S10753-015-0124-2. Baicalin Inhibits Lipopolysaccharide-Induced Inflammation Through Signaling Nf-??b Pathway In Hbe16 Airway Epithelial Cells.
Yu Q, Wang Z, Yang Q. J Dairy Sci. 2012 Oct;95(10):5580-7. Doi: 10.3168/Jds.2012-5540. Epub 2012 Aug 9. Lactobacillus Amylophilus D14 Protects Tight Junction From Enteropathogenic Bacteria Damage In Caco-2 Cells.
Li Ff, Shen J, Shen Hj, Zhang X, Cao R, Zhang Y, Qui Q, Lin Xx, Xie Yc, Zhang Lh, Jia Yl, Dong Xw, Jiang Jx, Bao Mj, Zhang S, Ma Wj, Wu Xm, Shen H, Xie Qm, Ke Y. J Immunol. 2012 Sep 15;189(6):3159-67. Doi: 10.4049/Jimmunol.1200197. Epub 2012 Aug 1...
He L, Zhang E, Shi J, Li X, Zhou K, Zhang Q, Le Ad, Tang X. Cancer Chemother Pharmacol. 2013 Mar;71(3):713-25. Doi: 10.1007/S00280-012-2063-Z. Epub 2013 Jan 6. (-)-Epigallocatechin-3-Gallate Inhibits Human Papillomavirus (Hpv)-16 Oncoprotein-Induc...
Bao Mh, Zhang Yw, Lou Xy, Xiao Y, Cheng Y, Zhou Hh. Can J Physiol Pharmacol. 2014 Apr;92(4):299-306. Doi: 10.1139/Cjpp-2013-0322. Epub 2014 Jan 10. Puerarin Protects Endothelial Cells From Oxidized Low Density Lipoprotein Induced Injuries Via The ...
Zhai X, Han Q, Shan Z, Qu X, Guo L, Zhou Y. Mol Med Rep. 2014 Dec;10(6):3052-8. Doi: 10.3892/Mmr.2014.2575. Epub 2014 Sep 18. Dual Specificity Phosphatase 6 Suppresses The Growth And Metastasis Of Prostate Cancer Cells.
Wang H, Zhuo Y, Hu X, Shen W, Zhang Y, Chu T. Biochem Biophys Res Commun. 2015 Mar 6;458(2):268-73. Doi: 10.1016/J.Bbrc.2015.01.097. Epub 2015 Feb 3. Cd147 Deficiency Blocks Il-8 Secretion And Inhibits Lung Cancer-Induced Osteoclastogenesis.
Pei X, Li M, Zhan J, Yu Y, Wei X, Guan L, Aydin H, Elson P, Zhou M, He H, Zhang H. Plos One. 2015 Apr 28;10(4):E0124338. Doi: 10.1371/Journal.Pone.0124338. Ecollection 2015. Enhanced Imp3 Expression Activates Nf-??b Pathway And Promotes Renal Cell ...
Xiao X, Gang Y, Wang H, Wang J, Zhao L, Xu L, Liu Z. Biochem Biophys Res Commun. 2015 Feb 6;457(2):221-6. Doi: 10.1016/J.Bbrc.2014.12.091. Epub 2014 Dec 27. Double-Stranded Rna Transcribed From Vector-Based Oligodeoxynucleotide Acts As Transcripti...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Zhang E, Feng X, Liu F, Zhang P, Liang J, Tang X. Plos One. 2014 Jul 24;9(7):E103440. Doi: 10.1371/Journal.Pone.0103440. Ecollection 2014. Roles Of Pi3K/Akt And C-Jun Signaling Pathways In Human Papillomavirus Type 16 Oncoprotein-Induced Hif-1??, V...
Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Biomaterials. 2012 Feb;33(5):1437-44. Doi: 10.1016/J.Biomaterials.2011.10.056. Epub 2011 Nov 10. The Enhancement Of Cancer Stem Cell Properties Of Mcf-7 Cells In 3D Collagen Scaffolds For...
Liu Lj, Zhang J, Xiao Zf, Dai B, Sun My, Chen L, Chen B. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):772-80. Doi: 10.1002/Jbm.B.33058. Epub 2013 Oct 21. Three-Dimensional Collagen Scaffold Enhances The Human Adenoid Cystic Carcinoma Cancer...
Ping C, Yongping Z, Minmin Q, Weiyan Y, Yaozong Y. Dig Dis Sci. 2010 Jun;55(6):1599-609. Doi: 10.1007/S10620-009-0909-Y. Epub 2009 Aug 13. Activated Protein C Improves The Severity Of Severe Acute Pancreatitis Via Up-Regulating The Expressions Of ...
Zhang H, Yin G, Jiang H, Zhang C. J Int Med Res. 2013 Aug;41(4):1179-86. Doi: 10.1177/0300060513488503. Epub 2013 Jun 14. High-Dose N-Acetylcysteine Decreases Silica-Induced Lung Fibrosis In The Rat.
Li Mc, Yang G, Zhou Xd, Tselluyko S, Perelman Jm. Int J Mol Med. 2014 Jan;33(1):83-90. Doi: 10.3892/Ijmm.2013.1535. Epub 2013 Oct 23. The Pathophysiological Mechanisms Underlying Mucus Hypersecretion Induced By Cold Temperatures In Cigarette Smoke...
Ren Dl, Li Yj, Hu Bb, Wang H, Hu B. J Pineal Res. 2015 May;58(4):452-60. Doi: 10.1111/Jpi.12230. Epub 2015 Mar 26. Melatonin Regulates The Rhythmic Migration Of Neutrophils In Live Zebrafish.
Liu L, Li C, Xiang J, Dong W, Cao Z. J Periodontal Res. 2013 Oct;48(5):615-22. Doi: 10.1111/Jre.12047. Epub 2013 Feb 27. Over-Expression And Potential Role Of Cyclophilin A In Human Periodontitis.
Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammatory...
Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig...
Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.
Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...
Hu Mm, Yang Q, Zhang J, Liu Sm, Zhang Y, Lin H, Huang Zf, Wang Yy, Zhang Xd, Zhong B, Shu Hb. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1509-14. Doi: 10.1073/Pnas.1318227111. Epub 2014 Jan 13. Trim38 Inhibits Tnf??- And Il-1??-Triggered Nf-??b Act...
Yang C, Ling H, Zhang M, Yang Z, Wang X, Zeng F, Wang C, Feng J. Mol Cells. 2011 Jun;31(6):531-8. Doi: 10.1007/S10059-011-1025-3. Epub 2011 Apr 20. Oxidative Stress Mediates Chemical Hypoxia-Induced Injury And Inflammation By Activating Nf-??b-Cox-...
Hu Y, Chen X, Duan H, Hu Y, Mu X. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. Doi: 10.3109/08923970902814129. Chinese Herbal Medicinal Ingredients Inhibit Secretion Of Il-6, Il-8, E-Selectin And Txb2 In Lps-Induced Rat Intestinal Microvascula...
Yang J, Yu Hm, Zhou Xd, Kolosov Vp, Perelman Jm. Mol Immunol. 2013 Jan;53(1-2):161-71. Doi: 10.1016/J.Molimm.2012.06.015. Epub 2012 Aug 30. Study On Trpv1-Mediated Mechanism For The Hypersecretion Of Mucus In Respiratory Inflammation.
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Cell Biochem Biophys. 2012 Apr;62(3):421-31. Doi: 10.1007/S12013-011-9324-Z. The Expression And Pharmacological Characterization Of Nicotinic Acetylcholine Receptor Subunits In Hbe16 Airway Epithelial Cells.
Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.
Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...
Zhang H, Xia X, Han F, Jiang Q, Rong Y, Song D, Wang Y. Mol Pharm. 2015 May 4;12(5):1648-61. Doi: 10.1021/Acs.Molpharmaceut.5B00069. Epub 2015 Apr 2. Cathelicidin-Bf, A Novel Antimicrobial Peptide From Bungarus Fasciatus, Attenuates Disease In A D...
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nap1 antibody, il 8 antibody, il8 antibody, il-8 antibody