Product Info Summary
SKU: | PA1467 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Interleukin-7 IL7 Antibody
SKU/Catalog Number
PA1467
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-7 IL7 Antibody catalog # PA1467. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-7 IL7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1467)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse IL7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1467 is reactive to IL7 in Human, Mouse
Applications
PA1467 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.187kDa
Background of IL-7
IL-7, Interleukin 7, is a protein that in humans is encoded by the IL-7 gene. IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. By the combination of approaches, IL-7 is located on 8q12-q13. IL-7 is critical for early T-cell development and homeostasis of naive and memory CD8-positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain is critical for early T-cell development and homeostasis of naive and memory CD8 -positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-IL7 antibody, PA1467, IHC(P)
IHC(P): Mouse Spleen Tissue
Click image to see more details
Figure 1. Western blot analysis of IL7 using anti-IL7 antibody (PA1467).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PA1467) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20KD. The expected band size for IL7 is at 20KD.
Protein Target Info & Infographic
Gene/Protein Information For IL7 (Source: Uniprot.org, NCBI)
Gene Name
IL7
Full Name
Interleukin-7
Weight
20.187kDa
Superfamily
IL-7/IL-9 family
Alternative Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF IL7 IL-7 interleukin 7 interleukin-7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL7, check out the IL7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interleukin-7 IL7 Antibody (PA1467)
Hello CJ!
No publications found for PA1467
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Interleukin-7 IL7 Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Interleukin-7 IL7 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Interleukin-7 IL7 Antibody
Question
My boss were satisfied with the WB result of your anti-IL7 antibody. However we have been able to see positive staining in pancreas secreted. using this antibody. Is that expected? Could you tell me where is IL7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
From what I have seen in literature, pancreas does express IL7. Generally IL7 expresses in secreted. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-13
Question
We bought anti-IL7 antibody for WB on sperm in the past. I am using human, and I plan to use the antibody for IHC-P next. My question regards examining sperm as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
I viewed the website and datasheets of our anti-IL7 antibody and I see that PA1467 has been validated on human in both WB and IHC-P. Thus PA1467 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-06
Question
We have seen staining in human sperm. What should we do? Is anti-IL7 antibody supposed to stain sperm positively?
K. Li
Verified customer
Asked: 2015-07-30
Answer
Based on literature sperm does express IL7. Based on Uniprot.org, IL7 is expressed in sperm, pancreas, among other tissues. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-07-30
Question
I was wanting to use using your anti-IL7 antibody for positive regulation of t cell differentiation studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
S. Dhar
Verified customer
Asked: 2014-08-29
Answer
Thank you for your inquiry. This PA1467 anti-IL7 antibody is tested on mouse spleen tissue. It is guaranteed to work for IHC-P, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-08-29
Question
We are currently using anti-IL7 antibody PA1467 for mouse tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
D. Collins
Verified customer
Asked: 2013-06-21
Answer
The anti-IL7 antibody (PA1467) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-21