Anti-IL7 Antibody Picoband™

IL-7 antibody

Boster Bio Anti-IL7 Antibody Picoband™ catalog # PB9521. Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9521
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-IL7 Antibody Picoband™

View all IL-7 Antibodies

SKU/Catalog Number

PB9521

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL7 Antibody Picoband™ catalog # PB9521. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9521)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IL7 recombinant protein (Position: D26-H177). Human IL7 shares 56.4% and 57% amino acid (aa) sequence identity with mouse and rat IL7, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9521 is reactive to IL7 in Human

Applications

PB9521 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

20.187kDa

Background of IL-7

IL-7, Interleukin 7, is a protein that in humans is encoded by the IL-7 gene. IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. By the combination of approaches, IL-7 is located on 8q12-q13. IL-7 is critical for early T-cell development and homeostasis of naive and memory CD8-positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain is critical for early T-cell development and homeostasis of naive and memory CD8 -positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IL7 (Source: Uniprot.org, NCBI)

Gene Name

IL7

Full Name

Interleukin-7

Weight

20.187kDa

Superfamily

IL-7/IL-9 family

Alternative Names

IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF IL7 IL-7 interleukin 7 interleukin-7

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL7, check out the IL7 Infographic

IL7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-IL7 Antibody Picoband™

Question

We were satisfied with the WB result of your anti-IL7 antibody. However we have seen positive staining in pancreas secreted. using this antibody. Is that expected? Could you tell me where is IL7 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

From what I have seen in literature, pancreas does express IL7. Generally IL7 expresses in secreted. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-05-01

Question

My lab would like using your anti-IL7 antibody for positive regulation of cell population proliferation studies. Has this antibody been tested with western blotting on k562 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

Thank you for your inquiry. This PB9521 anti-IL7 antibody is tested on k562 whole cell lysate. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-13

Question

We have been able to see staining in human pancreas. Do you have any suggestions? Is anti-IL7 antibody supposed to stain pancreas positively?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

From literature pancreas does express IL7. From Uniprot.org, IL7 is expressed in sperm, pancreas, among other tissues. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-27

Question

We purchased anti-IL7 antibody for WB on sperm a few months ago. I am using human, and We intend to use the antibody for ELISA next. I would like examining sperm as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

I viewed the website and datasheets of our anti-IL7 antibody and I see that PB9521 has been tested on human in both WB and ELISA. Thus PB9521 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-10

Question

We are currently using anti-IL7 antibody PB9521 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

The anti-IL7 antibody (PB9521) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-17

Order DetailsPrice
PB9521

100μg

$370
PB9521-10ug

10μg sample (liquid)

$99
PB9521-Biotin

100 μg Biotin conjugated

$570
PB9521-Cy3

100 μg Cy3 conjugated

$570
PB9521-Dylight488

100 μg Dylight488 conjugated

$570
PB9521-Dylight550

100 μg Dylight550 conjugated

$570
PB9521-Dylight594

100 μg Dylight594 conjugated

$570
PB9521-FITC

100 μg FITC conjugated

$570
PB9521-HRP

100 μg HRP conjugated

$570
PB9521-APC

100 μg APC conjugated

$670
PB9521-PE

100 μg PE conjugated

$670
PB9521-carrier-free

Carrier Free

$370

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