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Product Info Summary
| SKU: | A05404 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rabbit |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-IMP cyclohydrolase ATIC Monoclonal Antibody
SKU/Catalog Number
A05404
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IMP cyclohydrolase ATIC Monoclonal Antibody catalog # A05404. Tested in WB applications. This antibody reacts with Human, Rabbit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IMP cyclohydrolase ATIC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05404)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Immunogen
Purified recombinant human IMP cyclohydrolase protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05404 is reactive to ATIC in Human, Rabbit
Applications
A05404 is guaranteed for WB Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using IMP cyclohydrolase Monoclonal antibody against mouse colon, A549, A431, LNCAP, Jurkat, U2OS, rat liver, Mouse Kidney, Mouse Brain, HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ATIC (Source: Uniprot.org, NCBI)
Uniprot ID
P31939
Gene ID
471
Gene Name
ATIC
Full Name
Bifunctional purine biosynthesis protein PURH
Weight
64.616kDa
Superfamily
PurH family
Alternative Names
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase; 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR; AICARFT; AICARFT/IMPCHASE; bifunctional purine biosynthesis protein PURH; IMPCHASE; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PURHFLJ93545 ATIC AICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase bifunctional purine biosynthesis protein ATIC|5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase|AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme|AICAR transformylase/inosine monophosphate cyclohydrolase|AICARFT/IMPCHASE|bifunctional purine biosynthesis protein PURH|epididymis secretory sperm binding protein Li 70p|phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATIC, check out the ATIC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATIC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
Specific Publications For Anti-IMP cyclohydrolase ATIC Monoclonal Antibody (A05404)
No publications found for A05404
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-IMP cyclohydrolase ATIC Monoclonal Antibody
Question
My question regarding product A05404, anti-IMP cyclohydrolase Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Johnson
Verified customer
Asked: 2020-02-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05404 anti-IMP cyclohydrolase Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-05
Question
Is there a BSA free version of anti-IMP cyclohydrolase Monoclonal antibody A05404 available?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IMP cyclohydrolase Monoclonal antibody A05404 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-28
Question
We are currently using anti-IMP cyclohydrolase Monoclonal antibody A05404 for rabbit tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rabbit. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
The anti-IMP cyclohydrolase Monoclonal antibody (A05404) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-07
Question
Would A05404 anti-IMP cyclohydrolase Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
As indicated on the product datasheet, A05404 anti-IMP cyclohydrolase Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
