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SKU:MA1052
Clonality:Monoclonal
Clone Number:K36AC10
Application:IHC-P
Price: $200.00
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Overview

Product Name Anti-Insulin Antibody (Monoclonal, K36AC10)
Description Mouse IgG monoclonal antibody for Insulin, insulin (INS) detection. Tested with IHC-P in Human;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-Insulin Antibody (Monoclonal, K36AC10) K36AC10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1052)
Replacement Item This antibody may replace the following items: sc-168778|sc-168777|sc-101000|sc-390810|sc-398517|sc-514131|sc-514127 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rabbit, Rat
Application IHC-P

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human insulin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number K36AC10
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ins2
Protein Name Insulin
Molecular Weight 12339 MW
Protein Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Sequence Similarities Belongs to the insulin family.
Subcellular Localization Secreted.
Uniprot ID P01323
Alternative Names Insulin-2;Insulin-2 B chain;Insulin-2 A chain;Ins2;Ins-2;
Research Areas |cell biology|other antibodies|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Insulin

Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al.(1981) and Harper and Saunders(1981) assigned the insulin gene to 11p15.5 by in situ hybridization.

Anti-Insulin Antibody (Monoclonal, K36AC10) Images

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Anti-Insulin Antibody (Monoclonal, K36AC10)
Anti-Insulin antibody (monoclonal), MA1052, IHC(P)
IHC(P): Rat Pancreas Tissue
Anti-Insulin Antibody (Monoclonal, K36AC10)
Anti-Insulin antibody (monoclonal), MA1052, IHC(P)
IHC(P): Rat Pancreas Tissue
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Publications

Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...
Su X, Meng X, Sun C, Liu L, Su B. J Diabetes. 2011 Dec;3(4):309-16. Doi: 10.1111/J.1753-0407.2011.00153.X. Intramuscular Injection Of Soluble Receptor For Advanced Glycation Endproducts Expression Vector Prevents The Development Of Streptozotocin-...
Shen L, Liu X, Hou W, Yang G, Wu Y, Zhang R, Li X, Che H, Lu Z, Zhang Y, Liu X, Yao L. Cell Mol Life Sci. 2010 Apr;67(8):1371-81. Doi: 10.1007/S00018-010-0258-1. Epub 2010 Feb 3. Ndrg2 Is Highly Expressed In Pancreatic Beta Cells And Involved In P...
Chen Z, Yang S, He Y, Song C, Liu Y. Neural Regen Res. 2013 Jul 5;8(19):1756-64. Doi: 10.3969/J.Issn.1673-5374.2013.19.003. Effect Of Sericin On Diabetic Hippocampal Growth Hormone/Insulin-Like Growth Factor 1 Axis.
Xu Y, Ke H, Liang L, Wang X, Pei J, Du L. Horm Res Paediatr. 2011;76(1):35-43. Doi: 10.1159/000324566. Epub 2011 Mar 10. Peg-Rhgh Therapy For Small For Gestational Age Rats And Effects On Their Metabolic Parameters.
Adise S, Saliu A, Maldonado N, Khatri V, Cardoso L, Rodr??guez-Contreras A. J Neurosci. 2014 Mar 26;34(13):4528-33. Doi: 10.1523/Jneurosci.4188-13.2014. Effect Of Maternal Care On Hearing Onset Induced By Developmental Changes In The Auditory Perip...
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
Liang H, Yin B, Zhang H, Zhang S, Zeng Q, Wang J, Jiang X, Yuan L, Wang Cy, Li Z. Endocrinology. 2008 Jun;149(6):2943-51. Doi: 10.1210/En.2007-0978. Epub 2008 Mar 13. Blockade Of Tumor Necrosis Factor (Tnf) Receptor Type 1-Mediated Tnf-Alpha Signa...
Zhou J, Zhou S, Zeng S. Fundam Clin Pharmacol. 2013 Jun;27(3):279-87. Doi: 10.1111/J.1472-8206.2011.01022.X. Epub 2011 Dec 16. Experimental Diabetes Treated With Trigonelline: Effect On ?? Cell And Pancreatic Oxidative Parameters.
Wang X, Ni S, Xu Y, Liang L, Du L, Gu W. Endocr Res. 2012;37(3):97-109. Doi: 10.3109/07435800.2011.635621. Epub 2012 May 23. Effects Of Long-Term High-Fat/High-Energy And High-Protein Diets On Insulin And Ghrelin Expression In Developing Rats.
Hu N, Yuan L, Li Hj, Huang C, Mao Qm, Zhang Yy, Lin M, Sun Yq, Zhong Xy, Tang P, Lu X. Evid Based Complement Alternat Med. 2013;2013:180721. Doi: 10.1155/2013/180721. Epub 2013 May 29. Anti-Diabetic Activities Of Jiaotaiwan In Db/Db Mice By Augmen...
Gao Lh, Liu Q, Liu Sn, Chen Zy, Li Cn, Lei L, Sun Sj, Li Ly, Liu Jl, Shen Zf. J Ethnopharmacol. 2014;151(1):675-85. Doi: 10.1016/J.Jep.2013.11.024. Epub 2013 Nov 25. A Refined-Jinqi-Jiangtang Tablet Ameliorates Prediabetes By Reducing Insulin Resi...
Ding H, Zhu T, Yin X, Liu J, Zhang L, Bernier M, Zhao R. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):582-9. Doi: 10.1093/Abbs/Gmu034. Epub 2014 May 14. Pyrrolidine Dithiocarbamate Protects Pancreatic ??-Cells From Oxidative Damage Through R...
Chen Ll, Yang Wh, Zheng J, Hu X, Kong W, Zhang Hh. Nutr Metab (Lond). 2010 May 26;7:45. Doi: 10.1186/1743-7075-7-45. Effect Of Catch-Up Growth After Food Restriction On The Entero-Insular Axis In Rats.
Jiang H, Deng Xf, Duan Cm, Chen C, Xiang Jl, Lu Yl, Ma Qf. Lymphology. 2011 Mar;44(1):21-8. Somatostatin Receptors Sstr2 And Sstr5 Are Expressed In The Human Thoracic Duct.
Zhang Y, Ye C, Wang G, Gao Y, Tan K, Zhuo Z, Liu Z, Xia H, Yang D, Li P. Biomed Res Int. 2013;2013:526367. Doi: 10.1155/2013/526367. Epub 2013 May 27. Kidney-Targeted Transplantation Of Mesenchymal Stem Cells By Ultrasound-Targeted Microbubble Des...
Zhang W, Jin H, Hu Y, Yu Y, Li X, Ding Z, Kang Y, Wang B. Hum Gene Ther. 2010 Feb;21(2):171-8. Doi: 10.1089/Hum.2009.095. Protective Response Against Type 1 Diabetes In Nonobese Diabetic Mice After Coimmunization With Insulin And Dna Encoding Proi...
Liu Jj, Raynal S, Bailb?? D, Gausseres B, Carbonne C, Autier V, Movassat J, Kergoat M, Portha B. Biochim Biophys Acta. 2015 May;1852(5):980-91. Doi: 10.1016/J.Bbadis.2015.02.001. Epub 2015 Feb 9. Expression Of The Kynurenine Pathway Enzymes In The ...
Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agonis...
Han X, Qiu L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 2009 May;234(5):595-603. Doi: 10.3181/0812-Rm-359. Epub 2009 Feb 25. Transplantation Of Sertoli-Islet Cell Aggregates Formed By Microgravi...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is proinsulin antibody