Anti-Integrin alpha 3/ITGA3 Antibody
Rabbit IgG polyclonal antibody for Integrin alpha-3(ITGA3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Anti-Integrin alpha 3/ITGA3 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Integrin alpha 3/ITGA3 Antibody
See all ITGA3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Integrin alpha 3/ITGA3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Integrin alpha 3/ITGA3 information: Molecular Weight: 116612 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell membrane ; Lipid-anchor . Cell projection, invadopodium membrane ; Single-pass type I membrane protein . Cell projection, filopodium membrane ; Single-pass type I membrane protein . Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner; Tissue Specificity: Isoform 1 is widely expressed. Isoform 2 is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform 1 is strongly expressed on vascular smooth muscle cells, isoform 2 is detected only on endothelial vein cells.|
|Cite This Product||Anti-Integrin alpha 3/ITGA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1621-1)|
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|Anti-Integrin alpha 3/ITGA3 Antibody may replace the following items: sc 13545|sc 28665|sc 32237|sc 374242|sc 393290|sc 393298|sc 53354|sc 53355|sc 59965|sc 59966|sc 59967|sc 6587|sc 6588|sc 6592.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 3(1026-1040aa ALYEAKRQKAEMKSQ), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ITGA3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3675|
|Species Of This Entry||Human|
|Protein Name||Integrin alpha-3|
|Superfamily||integrin alpha chain family|
|Alternative Names||ITGA3|antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a|
|Post Tranlational Modifications||Glycosylation; Phosphorylation; Methylation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell projection, invadopodium membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner.|
|Gene Location||17q21.33, on Chromasome 17, gene sequence: NC_000017.11 (50056065..50090481)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ITGA3 (Source: Uniprot.org, NCBI)
|Protein Function||Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.|
|Tissue Specificity||Isoform 1 is widely expressed. Isoform 2 is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform 1 is strongly expressed on vascular smooth muscle cells, isoform 2 is detected only on endothelial vein cells.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Neoplasm Metastasis; Tissue Adhesions; Carcinoma; Malignant Squamous Cell Neoplasm; Cell Invasion; Cell Transformation, Neoplastic; Adenocarcinoma; Nephrotic Syndrome; Stomach Neoplasms; Metastatic Malignant Neoplasm To The Lymph Node; Neoplasm Invasiveness; Congenital Nephrotic Syndrome; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Lung; Carcinogenesis; Nervousness; Colorectal Cancer|
|Related Pathways||Cell Proliferation; Cell Adhesion; Regulation Of Biological Process; Reproductive Process; Skin Development; Inner Ear Development; Embryo Development; Blastocyst Formation; Axon Guidance; Cell Cycle; Multicellular Organismal Process; Wound Healing; Cell-cell Adhesion; Gastrulation; Response To Stimulus; Cellular Process; Dna Methylation; Glycosylation; Methylation; Keratinocyte Migration|
|Background||ITGA3(INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. ITGA3 is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. ITGA3 is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the alpha3beta1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit. A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.|
|Scientific References||PMID: 1655803 by Takada Y., et al. Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen.|
PMID: 1714443 by Tsuji T., et al. Identification of human galactoprotein b3, an oncogenic transformation-induced membrane glycoprotein, as VLA-3 alpha subunit: the primary structure of human integrin alpha 3.
Our Boster Quality Guarantee for Anti-Integrin alpha 3/ITGA3 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
WB: Rat Liver Tissue Lysate
IHC(P): Human Intestinal Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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