Anti-Integrin alpha 5/ITGA5 Antibody Picoband™

ITGA5 antibody

Boster Bio Anti-Integrin alpha 5/ITGA5 Antibody Picoband™ catalog # A01911. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: A01911
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Integrin alpha 5/ITGA5 Antibody Picoband™

View all ITGA5 Antibodies

SKU/Catalog Number

A01911

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Integrin alpha 5/ITGA5 Antibody Picoband™ catalog # A01911. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin alpha 5/ITGA5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01911)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01911 is reactive to ITGA5 in Human, Mouse, Rat

Applications

A01911 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

114.536kDa

Background of ITGA5

Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 2μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ITGA5 (Source: Uniprot.org, NCBI)

Gene Name

ITGA5

Full Name

Integrin alpha-5

Weight

114.536kDa

Superfamily

integrin alpha chain family

Alternative Names

Integrin alpha 5 beta 1; VLA-5 ITGA5 CD49e, FNRA, VLA-5, VLA5A integrin subunit alpha 5 integrin alpha-5|CD49 antigen-like family member E|ITGA5/SLC9B1 fusion|fibronectin receptor subunit alpha|fibronectin receptor, alpha polypeptide|fibronectin receptor, alpha subunit|integrin alpha-F|integrin, alpha 5 (fibronectin receptor, alpha polypeptide)|very late activation protein 5, alpha subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGA5, check out the ITGA5 Infographic

ITGA5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01911 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Calreticulin overexpression correlates with integrin-α5 and transforming growth factor-β1 expression in the atria of patients with rheumatic valvular disease and atrial fibrillation

Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion

A Small Physiological Electric Field Mediated Responses of Extravillous Trophoblasts Derived from HTR8/SVneo Cells: Involvement of Activation of Focal Adhesion Kinase Signaling

Characterization of Rat Hair Follicle Stem Cells Selected by Vario Magnetic Activated Cell Sorting System

Characterization of Rat Hair Follicle Stem Cells Selected by Vario Magnetic Activated Cell Sorting System

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8 Customer Q&As for Anti-Integrin alpha 5/ITGA5 Antibody Picoband™

Question

I am interested to purchase the Anti-Integrin alpha 5/ITGA5 Antibody Picoband to use it in cell culture to block the function of a5 integrin. What is the recommended concentration for this antibody in this kind of experiment? Does the product binds the cytoplasmic or extracellular domain?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

The product Anti-Integrin alpha 5/ITGA5 Antibody Picoband was used to inhibit VLA5-mediated functions in vitro. The application varies so we recommend to determine the optimum working dilution of the product that is appropriate for a specific need. Unfortunately we cannot provide information about the epitope and were the product binds. However, we can validate that the product sourced is external and no epitope mapping was performed, so the location of binding is unknown. But we can suggest that this clone reacts with the a5 chain of the integrin a5b1 fibronectin receptor on a variety of mouse cells and cell lines

Boster Scientific Support

Answered: 2019-12-17

Question

See attached the WB image, lot number and protocol we used for liver using anti-Integrin alpha 5/ITGA5 antibody A01911. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-06

Question

I see that the anti-Integrin alpha 5/ITGA5 antibody A01911 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-21

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Integrin alpha 5/ITGA5 antibody A01911. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-06-14

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-14

Question

Would anti-Integrin alpha 5/ITGA5 antibody A01911 work for IHC-P with liver?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

According to the expression profile of liver, ITGA5 is highly expressed in liver. So, it is likely that anti-Integrin alpha 5/ITGA5 antibody A01911 will work for IHC-P with liver.

Boster Scientific Support

Answered: 2018-05-08

Question

I am interested in to test anti-Integrin alpha 5/ITGA5 antibody A01911 on mouse liver for research purposes, then I may be interested in using anti-Integrin alpha 5/ITGA5 antibody A01911 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

O. Moore

Verified customer

Asked: 2017-09-06

Answer

The products we sell, including anti-Integrin alpha 5/ITGA5 antibody A01911, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-06

Question

Is a blocking peptide available for product anti-Integrin alpha 5/ITGA5 antibody (A01911)?

Verified Customer

Verified customer

Asked: 2017-06-16

Answer

We do provide the blocking peptide for product anti-Integrin alpha 5/ITGA5 antibody (A01911). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-16

Question

I am looking for the function blocking antibody for integrin alpha 5 to use in human cells. Is your product Anti-Integrin alpha 5/ITGA5 Antibody Picoband would be suitable for it?

Verified Customer

Verified customer

Asked: 2014-08-29

Answer

Yes. We would recommend to use Anti-Integrin alpha 5/ITGA5 Antibody Picoband, since it is the only anti-integrin alpha 5 antibody listed in our catalogue whose inhibitory activity has been previously tested. Thus, it is suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T and B lymphocytes on fibronectin. For your further reference please check this on PUBMID: 16548586 ; Mardilovich A et al. Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized biomaterials. Langmuir 22:3259-64 (2006)

Boster Scientific Support

Answered: 2014-08-29

Order DetailsPrice
A01911

100μg

$370
A01911-10ug

10μg sample (liquid)

$99
A01911-Biotin

100 μg Biotin conjugated

$570
A01911-Cy3

100 μg Cy3 conjugated

$570
A01911-Dylight488

100 μg Dylight488 conjugated

$570
A01911-Dylight550

100 μg Dylight550 conjugated

$570
A01911-Dylight594

100 μg Dylight594 conjugated

$570
A01911-FITC

100 μg FITC conjugated

$570
A01911-HRP

100 μg HRP conjugated

$570
A01911-APC

100 μg APC conjugated

$670
A01911-PE

100 μg PE conjugated

$670
A01911-carrier-free

Carrier Free

$370

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A01911
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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