|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Integrin Alpha E Antibody|
|Description||Rabbit IgG polyclonal antibody for Integrin alpha-E(ITGAE) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Integrin Alpha E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1622)|
|Replacement Item||This antibody may replace the following items: sc-19981|sc-28662|sc-53357|sc-6606|sc-6607 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Predicted Species||Horse, Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha E(1153-1168aa KRKYQQLNLESIRKAQ), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Integrin alpha-E|
|Molecular Weight||130159 MW|
|Protein Function||Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.|
|Tissue Specificity||Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.|
|Sequence Similarities||Belongs to the integrin alpha chain family.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Integrin alpha-E;HML-1 antigen;Integrin alpha-IEL;Mucosal lymphocyte 1 antigen;CD103;Integrin alpha-E light chain;Integrin alpha-E heavy chain;ITGAE;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cell adhesion|integrins| immunology|cell type markers| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage|adaptive immunity|regulatory t cells||
Background for Integrin alpha-E
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Integrin Alpha E Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Integrin Alpha E Antibody Images
Click the images to enlarge.
IHC(P): Human Rectal Cancer Tissue
All lanes: Anti Integrin alpha E (PA1622) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
IHC(P): Rat Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,