Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody

ITGB1 antibody

Boster Bio Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody catalog # M00772-2. Tested in WB, IHC applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: M00772-2
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody

View all ITGB1 Antibodies

SKU/Catalog Number

M00772-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody catalog # M00772-2. Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00772-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFI-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Integrin beta 1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00772-2 is reactive to ITGB1 in Human

Applications

M00772-2 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

88.415kDa

Background of ITGB1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For ITGB1 (Source: Uniprot.org, NCBI)

Gene Name

ITGB1

Full Name

Integrin beta-1

Weight

88.415kDa

Superfamily

integrin beta chain family

Alternative Names

CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB integrin subunit beta 1 integrin beta-1|glycoprotein IIa|integrin VLA-4 beta subunit|integrin beta 1|integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)|very late activation protein, beta polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGB1, check out the ITGB1 Infographic

ITGB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00772-2 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation

Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells

Have you used Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody?

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7 Customer Q&As for Anti-Integrin beta 1 ITGB1 Rabbit Monoclonal Antibody

Question

Will anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 work for IHC with skin?

C. Johnson

Verified customer

Asked: 2019-11-21

Answer

According to the expression profile of skin, ITGB1 is highly expressed in skin. So, it is likely that anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 will work for IHC with skin.

Boster Scientific Support

Answered: 2019-11-21

Question

I see that the anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-22

Question

My lab would like to test anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 on human skin for research purposes, then I may be interested in using anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

The products we sell, including anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-10

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2. Let me know if you need anything else.

A. Mitchell

Verified customer

Asked: 2019-04-25

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-25

Question

Do you have a BSA free version of anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 available?

L. Mangal

Verified customer

Asked: 2018-11-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-11-13

Question

Will anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 work on zebrafish IHC with skeletal muscle?

D. Wu

Verified customer

Asked: 2018-08-31

Answer

Our lab technicians have not validated anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Integrin beta 1 Rabbit Monoclonal antibody M00772-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish skeletal muscle in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-31

Question

I was wanting to use your anti-Integrin beta 1 Rabbit Monoclonal antibody for IHC for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?

Z. Wu

Verified customer

Asked: 2015-06-16

Answer

As indicated on the product datasheet, M00772-2 anti-Integrin beta 1 Rabbit Monoclonal antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-06-16

Order DetailsPrice
M00772-2

100μL

$370
M00772-2-30ul

30μL sample

$99
M00772-2-carrier-free

Carrier Free

$370

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M00772-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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