Anti-Integrin beta 4/ITGB4 Antibody
Rabbit IgG polyclonal antibody for Integrin beta-4(ITGB4) detection. Tested with WB in Human.
Anti-Integrin beta 4/ITGB4 Antibody Info At A Glance
|Product Name||Anti-Integrin beta 4/ITGB4 Antibody
See all Integrin beta 4/CD104 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CD104/ITGB4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CD104/ITGB4 information: Molecular Weight: 202167 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Colocalizes with DST at the leading edge of migrating keratinocytes; Tissue Specificity: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.|
|Cite This Product||Anti-Integrin beta 4/ITGB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1702-2)|
|Similar Products From
|Anti-Integrin beta 4/ITGB4 Antibody may replace the following items: sc 162284|sc 162283|sc 162282.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Integrin beta 4(422-437aa HVCQLPEDQKGNIHLK).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ITGB4 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3691|
|Species Of This Entry||Human|
|Protein Name||Integrin beta-4|
|Superfamily||integrin beta chain family|
|Alternative Names||Integrin beta 4/CD104|CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4|
|Post Tranlational Modifications||Disulfide bond; Glycoprotein; Lipoprotein; Palmitate; Phosphoprotein|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Colocalizes with DST at the leading edge of migrating keratinocytes.|
|Gene Location||17q25.1, on Chromasome 17, gene sequence: NC_000017.11 (75721339..75757819)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ITGB4 (Source: Uniprot.org, NCBI)
|Protein Function||Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).|
|Tissue Specificity||Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.|
|Interacting Proteins||ARRDC3; ITGA6; PLEC; PTK2; S1PR2; S1PR3|
|Research Areas||Adaptive Immunity; Immunology; Signal Transduction|
|Related Diseases||Epidermolysis Bullosa; Atresia; Malignant Neoplasms; Junctional Epidermolysis Bullosa; Bulla; Tissue Adhesions; Epidermolysis Bullosa Junctionalis With Pyloric Atresia; Neoplasms; Neoplasm Metastasis; Carcinoma; Cell Invasion; Malignant Neoplasm Of Breast; Malignant Squamous Cell Neoplasm; Skin Diseases, Genetic; Dermatologic Disorders; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Lung|
|Related Pathways||Cell Adhesion; Cell Proliferation; Cell Migration; Immune Response; Translation; Pathogenesis; Cell Cycle; Invasive Growth; Cell Growth; Angiogenesis; Transport; Cell Activation; Neutrophil Extravasation; Adaptive Immune Response; Regulation Of Gene Expression; Myelination; Mesenchymal Cell Migration; Inflammatory Response; T Cell Proliferation; Secretory Pathway|
|Background||ITGB4(Integrin, beta-4), also known as CD104(Cluster of Differentiation 104), is a human gene. The gene encodes the integrin beta 4 subunits, a receptor for the laminins. This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. The ITGB4 gene is mapped on 17q25.1. Using expression profiling, Yang et al. found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells. They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines. The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III(FNIII) repeats, residing in 2 pairs separated by a connecting segment. Vidal et al. found compound heterozygosity for mutations in the ITGB4 gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia.|
|Scientific References||PMID: 2311577 by Suzuki S., et al. Amino acid sequence of a novel integrin beta 4 subunit and primary expression of the mRNA in epithelial cells.|
PMID: 2311578 by Hogervorst F., et al. Cloning and sequence analysis of beta-4 cDNA: an integrin subunit that contains a unique 118 kd cytoplasmic domain.
Our Boster Quality Guarantee for Anti-Integrin beta 4/ITGB4 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti ITGB4 (PA1702-2) at 0.5ug/ml
WB: A431 Whole Cell Lysate at 40ug
Predicted bind size: 200KD
Observed bind size: 200KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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