Data & Images
|Product Name||Anti-Integrin Beta 4 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Integrin beta-4(ITGB4) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Integrin Beta 4 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9007)|
|Replacement Item||This antibody may replace the following items: sc-13127|sc-13543|sc-135950|sc-377523|sc-514252|sc-514426|sc-55514|sc-6628|sc-6629|sc-9090 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Integrin beta-4|
|Molecular Weight||202167 MW|
|Protein Function||Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. .|
|Tissue Specificity||Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.|
|Sequence Similarities||Belongs to the integrin beta chain family.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Colocalizes with DST at the leading edge of migrating keratinocytes.|
|Alternative Names||Integrin beta-4;GP150;CD104;ITGB4;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cell adhesion|integrins||
Background for Integrin beta-4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Integrin Beta 4 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Integrin Beta 4 Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Placenta Tissue
All lanes: Anti-ITGB4(PB9007) at 0.5ug/ml
WB: Recombinant Human Integrin beta4 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,