Anti-Interferon gamma/IFNG Antibody Picoband™

IFN-gamma antibody

Boster Bio Anti-Interferon gamma/IFNG Antibody Picoband™ catalog # A00393-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: A00393-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Interferon gamma/IFNG Antibody Picoband™

View all IFN-gamma Antibodies

SKU/Catalog Number

A00393-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interferon gamma/IFNG Antibody Picoband™ catalog # A00393-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interferon gamma/IFNG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00393-3 is reactive to IFNG in Human, Mouse, Rat

Applications

A00393-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

19.348kDa

Background of IFN-gamma

IInterferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)

Gene Name

IFNG

Full Name

Interferon gamma

Weight

19.348kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IFNG, check out the IFNG Infographic

IFNG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00393-3 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The expression and significance of IL-6, IFN-γ, SM22α, and MMP-2 in rat model of aortic dissection.

Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation

Anti‐arthritis activity of cationic materials

Bu-Shen-Fang-Chuan formula attenuates T-lymphocytes recruitment in the lung of rats with COPD through suppressing CXCL9/CXCL10/CXCL11-CXCR3 axis

Quercetin inhibiting the PD-1/PD-L1 interaction for immune-enhancing cancer chemopreventive agent

Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation

Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

Li Xl, Dou Yc, Liu Y, Shi Cw, Cao Ll, Zhang Xq, Zhu J, Duan Rs. Cell Immunol. 2011;271(2):455-61. Doi: 10.1016/J.Cellimm.2011.08.015. Epub 2011 Aug 23. Atorvastatin Ameliorates Experimental Autoimmune Neuritis By Decreased Th1/Th17 Cytokines And U...

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16 Customer Q&As for Anti-Interferon gamma/IFNG Antibody Picoband™

Question

We have been able to see staining in rat blood. Do you have any suggestions? Is anti-Interferon gamma/IFNG antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

According to literature blood does express IFNG. According to Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-16

Question

Will A00393-3 anti-Interferon gamma/IFNG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

It shows on the product datasheet, A00393-3 anti-Interferon gamma/IFNG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-17

Question

Is this A00393-3 anti-Interferon gamma/IFNG antibody reactive to the isotypes of IFNG?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

The immunogen of A00393-3 anti-Interferon gamma/IFNG antibody is E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-17

Question

Would anti-Interferon gamma/IFNG antibody A00393-3 work for WB with leukocyte?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

According to the expression profile of leukocyte, IFNG is highly expressed in leukocyte. So, it is likely that anti-Interferon gamma/IFNG antibody A00393-3 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2019-12-11

Question

I am interested in using your anti-Interferon gamma/IFNG antibody for regulation of growth studies. Has this antibody been tested with western blotting on placenta tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

Thanks for your inquiry. This A00393-3 anti-Interferon gamma/IFNG antibody is validated on human sarcoma tissue, hela whole cell lysates, placenta tissue, rat lung tissue, brain tissue, mouse ovary tissue, cancer tissue, tonsil tissue, testis tissue. It is guaranteed to work for IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-19

Question

I see that the anti-Interferon gamma/IFNG antibody A00393-3 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-23

Question

Can you help my question with product A00393-3, anti-Interferon gamma/IFNG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-12

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00393-3 anti-Interferon gamma/IFNG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-12

Question

We need to test anti-Interferon gamma/IFNG antibody A00393-3 on rat leukocyte for research purposes, then I may be interested in using anti-Interferon gamma/IFNG antibody A00393-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Anderson

Verified customer

Asked: 2018-04-24

Answer

The products we sell, including anti-Interferon gamma/IFNG antibody A00393-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-24

Question

I was wanting to use your anti-Interferon gamma/IFNG antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukocyte identification?

J. Anderson

Verified customer

Asked: 2018-04-19

Answer

As indicated on the product datasheet, A00393-3 anti-Interferon gamma/IFNG antibody has been validated for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-04-19

Question

Our lab were happy with the WB result of your anti-Interferon gamma/IFNG antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?

G. Li

Verified customer

Asked: 2018-02-28

Answer

According to literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-02-28

Question

Is a blocking peptide available for product anti-Interferon gamma/IFNG antibody (A00393-3)?

Verified Customer

Verified customer

Asked: 2017-12-27

Answer

We do provide the blocking peptide for product anti-Interferon gamma/IFNG antibody (A00393-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-27

Question

Do you have a BSA free version of anti-Interferon gamma/IFNG antibody A00393-3 available?

C. Zhang

Verified customer

Asked: 2016-02-26

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma/IFNG antibody A00393-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-02-26

Question

We are currently using anti-Interferon gamma/IFNG antibody A00393-3 for rat tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

C. Kulkarni

Verified customer

Asked: 2015-11-09

Answer

The anti-Interferon gamma/IFNG antibody (A00393-3) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-11-09

Question

See below the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma/IFNG antibody A00393-3. Please let me know if you require anything else.

K. Brown

Verified customer

Asked: 2015-10-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-10-16

Question

We purchased anti-Interferon gamma/IFNG antibody for IHC-P on blood in a previous experiment. I am using mouse, and We are going to use the antibody for WB next. My lab would like examining blood as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?

R. Li

Verified customer

Asked: 2015-08-19

Answer

I have checked the website and datasheets of our anti-Interferon gamma/IFNG antibody and I see that A00393-3 has been tested on mouse in both IHC-P and WB. Thus A00393-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-08-19

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Interferon gamma/IFNG antibody A00393-3. Let me know if you need anything else.

M. Patel

Verified customer

Asked: 2013-02-12

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-02-12

Order DetailsPrice
A00393-3

100μg

$370
A00393-3-10ug

10μg sample (liquid)

$99
A00393-3-Biotin

100 μg Biotin conjugated

$570
A00393-3-Cy3

100 μg Cy3 conjugated

$570
A00393-3-Dylight488

100 μg Dylight488 conjugated

$570
A00393-3-Dylight550

100 μg Dylight550 conjugated

$570
A00393-3-Dylight594

100 μg Dylight594 conjugated

$570
A00393-3-FITC

100 μg FITC conjugated

$570
A00393-3-HRP

100 μg HRP conjugated

$570
A00393-3-APC

100 μg APC conjugated

$670
A00393-3-PE

100 μg PE conjugated

$670
A00393-3-carrier-free

Carrier Free

$370

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