Anti-Interferon gamma Biotinylated Ifng Antibody

Boster Bio Anti-Interferon gamma Biotinylated Ifng Antibody catalog # A00393-1-Biotin. Tested in ELISA applications. This antibody reacts with Mouse. Cited in 3 publication(s).

Product Info Summary

SKU: A00393-1-Biotin
Size: 50μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA

Product Name

Anti-Interferon gamma Biotinylated Ifng Antibody

See all IFN-gamma products

SKU/Catalog Number

A00393-1-Biotin

Size

50μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interferon gamma Biotinylated Ifng Antibody catalog # A00393-1-Biotin. Tested in ELISA applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interferon gamma Biotinylated Ifng Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393-1-Biotin)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse Interferon gamma recombinant protein (Position: M1-C155).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00393-1-Biotin is reactive to Ifng in Mouse

Applications

A00393-1-Biotin is guaranteed for ELISA Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Ifng (Source: Uniprot.Org, NCBI)

Uniprot ID

P01580

Gene ID

15978

Gene Name

Ifng

Full Name

Interferon gamma

Weight

17.907kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma Ifng|IFN-g, If, If2f, Ifg|interferon gamma|interferon gamma|IFN-gamma|gamma interferon|interferon 2f

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ifng, check out the Ifng Infographic

Ifng infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00393-1-Biotin has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation

Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

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14 Customer Q&As for Anti-Interferon gamma Biotinylated Ifng Antibody

Question

Is this A00393-1-Biotin anti-Interferon gamma Biotinylated antibody reactive to the isotypes of IFNG?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

The immunogen of A00393-1-Biotin anti-Interferon gamma Biotinylated antibody is E. coli-derived mouse Interferon gamma recombinant protein (Position: M1-C155). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-20

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Interferon gamma Biotinylated antibody A00393-1-Biotin. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

My boss were well pleased with the WB result of your anti-Interferon gamma Biotinylated antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

Based on literature, blood does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-08-13

Question

Is there a BSA free version of anti-Interferon gamma Biotinylated antibody A00393-1-Biotin available?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Interferon gamma Biotinylated antibody A00393-1-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-04

Question

See attached the WB image, lot number and protocol we used for blood using anti-Interferon gamma Biotinylated antibody A00393-1-Biotin. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-01-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-07

Question

Is a blocking peptide available for product anti-Interferon gamma Biotinylated antibody (A00393-1-Biotin)?

Verified Customer

Verified customer

Asked: 2018-07-12

Answer

We do provide the blocking peptide for product anti-Interferon gamma Biotinylated antibody (A00393-1-Biotin). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-07-12

Question

I am interested in to test anti-Interferon gamma Biotinylated antibody A00393-1-Biotin on mouse blood for research purposes, then I may be interested in using anti-Interferon gamma Biotinylated antibody A00393-1-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-06-05

Answer

The products we sell, including anti-Interferon gamma Biotinylated antibody A00393-1-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-06-05

Question

My question regarding product A00393-1-Biotin, anti-Interferon gamma Biotinylated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-11-17

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00393-1-Biotin anti-Interferon gamma Biotinylated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-11-17

Question

I see that the anti-Interferon gamma Biotinylated antibody A00393-1-Biotin works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-29

Question

We have seen staining in mouse leukocyte. Do you have any suggestions? Is anti-Interferon gamma Biotinylated antibody supposed to stain leukocyte positively?

M. Jackson

Verified customer

Asked: 2017-02-21

Answer

According to literature leukocyte does express IFNG. According to Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-02-21

Question

I was wanting to use your anti-Interferon gamma Biotinylated antibody for ELISA for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?

E. Kulkarni

Verified customer

Asked: 2016-11-21

Answer

It shows on the product datasheet, A00393-1-Biotin anti-Interferon gamma Biotinylated antibody has been tested for ELISA on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-11-21

Question

Does A00393-1-Biotin anti-Interferon gamma Biotinylated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Thomas

Verified customer

Asked: 2016-08-31

Answer

As indicated on the product datasheet, A00393-1-Biotin anti-Interferon gamma Biotinylated antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-08-31

Question

We are currently using anti-Interferon gamma Biotinylated antibody A00393-1-Biotin for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on monkey tissues as well?

D. Carter

Verified customer

Asked: 2015-07-14

Answer

The anti-Interferon gamma Biotinylated antibody (A00393-1-Biotin) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-07-14

Question

Does anti-Interferon gamma Biotinylated antibody A00393-1-Biotin work for ELISA with blood?

R. Mitchell

Verified customer

Asked: 2013-05-14

Answer

According to the expression profile of blood, IFNG is highly expressed in blood. So, it is likely that anti-Interferon gamma Biotinylated antibody A00393-1-Biotin will work for ELISA with blood.

Boster Scientific Support

Answered: 2013-05-14

Size

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A00393-1-Biotin
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