|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-Involucrin Antibody (Monoclonal, SY5)|
|Description||Mouse IgG monoclonal antibody for Involucrin, involucrin (IVL) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;pig. No cross reactivity with other proteins.|
|Cite This Product||Anti-Involucrin Antibody (Monoclonal, SY5) SY5 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1053)|
|Replacement Item||This antibody may replace the following items: sc-15223|sc-15225|sc-21748|sc-28557|sc-398221|sc-398952|sc-53361|sc-56555 from Santa Cruz Biotechnology.|
|Validated Species||Human, Pig|
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||67021 MW|
|Protein Function||Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.|
|Tissue Specificity||Keratinocytes of epidermis and other stratified squamous epithelia.|
|Subcellular Localization||Cytoplasm. Constituent of the scaffolding of the cornified envelope.|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|structures|keratin| cancer|tumor biomarkers||
Background for Involucrin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Involucrin Antibody (Monoclonal, SY5) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, By Heat
Immunocytochemistry , 1μg/ml, Human, pig, -
Immunohistochemistry(Frozen Section), 0.4-1μg/ml, Human, pig, -
Western blot, 2μg/ml, Human, pig
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Involucrin Antibody (Monoclonal, SY5) Images
Click the images to enlarge.
IHC(P): Human Lung Cancer Tissue