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SKU:MA1053
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:SY5
Application:IHC, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Involucrin Antibody (Monoclonal, SY5)
SKU/Catalog Number MA1053
Description Mouse IgG monoclonal antibody for Involucrin, involucrin (IVL) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;pig. No cross reactivity with other proteins.
Cite This Product Anti-Involucrin Antibody (Monoclonal, SY5) SY5 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1053)
Replacement Item This antibody may replace the following items: sc-15223|sc-15225|sc-21748|sc-28557|sc-398221|sc-398952|sc-53361|sc-56555 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Pig
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human involucrin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number SY5
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ivl
Protein Name Involucrin
Molecular Weight 67021 MW
Protein Function Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Tissue Specificity Keratinocytes of epidermis and other stratified squamous epithelia.
Subcellular Localization Cytoplasm. Constituent of the scaffolding of the cornified envelope.
Uniprot ID P48998
Alternative Names Involucrin;Ivl;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|structures|keratin| cancer|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Involucrin

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Anti-Involucrin Antibody (Monoclonal, SY5) Images

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Anti-Involucrin Antibody (Monoclonal, SY5)
Anti- -Involucrin antibody, MA1053, IHC(P)
IHC(P): Human Lung Cancer Tissue
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.