Product Info Summary
SKU: | M03051 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-IPO-38 HMGN1 Monoclonal Antibody
View all HMGN1/HMG14 Antibodies
SKU/Catalog Number
M03051
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IPO-38 HMGN1 Monoclonal Antibody (Catalog # M03051). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-IPO-38 HMGN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03051)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: IPO-38 IgM kappa
Isotype
IgM kappa
Immunogen
A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03051 is reactive to HMGN1 in Human
Applications
M03051 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
10.659kDa
Background of HMGN1/HMG14
The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: 10 µg/mL
WB: 1:500- 1:2,000
Validation Images & Assay Conditions
Click image to see more details
IPO-38 was detected in paraffin-embedded sections of human skin tissue using mouse anti-IPO-38 monoclonal antibody (Catalog # M03051) at 10 μg/mL. Staining: IPO-38 as precipitated red signal with hematoxylin purple nuclear counterstain.
Protein Target Info & Infographic
Gene/Protein Information For HMGN1 (Source: Uniprot.org, NCBI)
Gene Name
HMGN1
Full Name
Non-histone chromosomal protein HMG-14
Weight
10.659kDa
Superfamily
HMGN family
Alternative Names
FLJ31471; High mobility group nucleosome-binding domain-containing protein 1; high-mobility group (nonhistone chromosomal) protein 14; high-mobility group nucleosome binding 1; high-mobility group nucleosome binding domain 1; HMG14; HMG14FLJ27265; HMGN1; MGC104230; MGC117425; non-histone chromosomal protein HMG-14; nonhistone chromosomal protein HMG-14 HMGN1 HMG14 high mobility group nucleosome binding domain 1 non-histone chromosomal protein HMG-14|high mobility group nucleosome-binding domain-containing protein 1|high-mobility group (nonhistone chromosomal) protein 14|high-mobility group nucleosome binding 1|nonhistone chromosomal protein HMG-14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMGN1, check out the HMGN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMGN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IPO-38 HMGN1 Monoclonal Antibody (M03051)
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No publications found for M03051
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-IPO-38 HMGN1 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-IPO-38 Monoclonal antibody (M03051)?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
We do provide the blocking peptide for product anti-IPO-38 Monoclonal antibody (M03051). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-10
Question
I was wanting to use to test anti-IPO-38 Monoclonal antibody M03051 on human t-cell for research purposes, then I may be interested in using anti-IPO-38 Monoclonal antibody M03051 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
The products we sell, including anti-IPO-38 Monoclonal antibody M03051, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-21
Question
I have a question about product M03051, anti-IPO-38 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Dhar
Verified customer
Asked: 2015-03-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03051 anti-IPO-38 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-30
Question
Please see the WB image, lot number and protocol we used for t-cell using anti-IPO-38 Monoclonal antibody M03051. Please let me know if you require anything else.
K. Singh
Verified customer
Asked: 2014-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-08
Question
We are currently using anti-IPO-38 Monoclonal antibody M03051 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
G. Krishna
Verified customer
Asked: 2013-03-20
Answer
The anti-IPO-38 Monoclonal antibody (M03051) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-20