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SKU:PA2269
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-IRS1 Antibody
Description Rabbit IgG polyclonal antibody for Insulin receptor substrate 1(IRS1) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-IRS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2269)
Replacement Item This antibody may replace the following items: sc-101709|sc-101710|sc-101711|sc-101712|sc-17192|sc-17192-R|sc-17193-R|sc-17194|sc-17194-R|sc-17195-R|sc-17196|sc-17196-R|sc-17197|sc-17197-R|sc-17199-R|sc-17200|sc-17200-R|sc-17201|sc-17201-R|sc-17202-R|sc-22300-R|sc-33028|sc-33956|sc-33957|sc-515017|sc-559|sc-559-G|sc-560|sc-7200|sc-8038 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse IRS1(106-122aa WYQALLQLHNRAKAHHD), identical to the related rat sequence, and different from the related human sequence by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Irs1
Protein Name Insulin receptor substrate 1
Molecular Weight 130723 MW
Protein Function May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). .
Tissue Specificity Expressed in osteoblasts, but not in osteoclasts. .
Sequence Similarities Contains 1 IRS-type PTB domain.
Uniprot ID P35569
Alternative Names Insulin receptor substrate 1;IRS-1;Irs1;Irs-1;
Research Areas |tags & cell markers|cell type markers|tumor associated| signal transduction|adapters|cytoplasmic|growth factors/hormones|insulin / insulin-like| neuroscience|neurology process|metabolism| cancer| cardiovascular|atherosclerosis|diabetes associated| metabolism|types of disease|diabetes|obesity|heart disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Insulin receptor substrate 1

Insulin receptor substrate 1(IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1(IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also plays important biological function for both metabolic and mitogenic(growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.

Anti-IRS1 Antibody Images

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Anti-IRS1 Antibody
Anti-IRS1 antibody, PA2269, All Western blotting
All lanes: Anti-IRS1(PA2269) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 132KD
Observed bind size: 132KD
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Publications

Mi L, Chen Y, Zheng X, Li Y, Zhang Q, Mo D, Yang G. J Cell Biochem. 2015 Jul;116(7):1195-204. Doi: 10.1002/Jcb.25065. Microrna-139-5P Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch And Irs1/Pi3K/Akt Insulin Signaling Pathways.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to irs 1 antibody, irs1 antibody, irs-1 antibody