Anti-Islet 1 Rabbit Monoclonal Antibody

Islet-1 antibody

Boster Bio Anti-Islet 1 Rabbit Monoclonal Antibody catalog # M02969. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M02969
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IP, IF, ICC, WB

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Product Name

Anti-Islet 1 Rabbit Monoclonal Antibody

View all Islet-1 Antibodies

SKU/Catalog Number

M02969

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Islet 1 Rabbit Monoclonal Antibody catalog # M02969. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Islet 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02969)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EHO-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Islet 1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02969 is reactive to ISL1 in Human, Mouse

Applications

M02969 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

39.036kDa

Background of Islet-1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ISL1 (Source: Uniprot.org, NCBI)

Gene Name

ISL1

Full Name

Insulin gene enhancer protein ISL-1

Weight

39.036kDa

Alternative Names

insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain, (islet-1); ISL1; Isl-1; Islet1; Islet-1 ISL1 ISLET1, Isl-1 ISL LIM homeobox 1 insulin gene enhancer protein ISL-1|islet-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ISL1, check out the ISL1 Infographic

ISL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ISL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Islet 1 Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-Islet 1 Rabbit Monoclonal antibody for IP for mouse pancreatic islet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse pancreatic islet identification?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

As indicated on the product datasheet, M02969 anti-Islet 1 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse pancreatic islet in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-17

Question

I see that the anti-Islet 1 Rabbit Monoclonal antibody M02969 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreatic islet using anti-Islet 1 Rabbit Monoclonal antibody M02969. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-01

Question

Does anti-Islet 1 Rabbit Monoclonal antibody M02969 work for IP with pancreatic islet?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

According to the expression profile of pancreatic islet, ISL1 is highly expressed in pancreatic islet. So, it is likely that anti-Islet 1 Rabbit Monoclonal antibody M02969 will work for IP with pancreatic islet.

Boster Scientific Support

Answered: 2019-10-29

Question

My question regards to test anti-Islet 1 Rabbit Monoclonal antibody M02969 on mouse pancreatic islet for research purposes, then I may be interested in using anti-Islet 1 Rabbit Monoclonal antibody M02969 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Zhang

Verified customer

Asked: 2019-09-20

Answer

The products we sell, including anti-Islet 1 Rabbit Monoclonal antibody M02969, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-20

Question

Is there a BSA free version of anti-Islet 1 Rabbit Monoclonal antibody M02969 available?

W. Moore

Verified customer

Asked: 2019-09-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Islet 1 Rabbit Monoclonal antibody M02969 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-05

Question

Our lab were well pleased with the WB result of your anti-Islet 1 Rabbit Monoclonal antibody. However we have observed positive staining in forebrain nucleus. using this antibody. Is that expected? Could you tell me where is ISL1 supposed to be expressed?

M. Li

Verified customer

Asked: 2019-05-09

Answer

According to literature, forebrain does express ISL1. Generally ISL1 expresses in nucleus. Regarding which tissues have ISL1 expression, here are a few articles citing expression in various tissues:
Pancreatic islet, Pubmed ID: 7912209
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-09

Question

Is this M02969 anti-Islet 1 Rabbit Monoclonal antibody reactive to the isotypes of ISL1?

M. Zhao

Verified customer

Asked: 2018-10-22

Answer

The immunogen of M02969 anti-Islet 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Islet 1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-10-22

Question

We ordered your anti-Islet 1 Rabbit Monoclonal antibody for IP on pancreatic islet in the past. I am using mouse, and I plan to use the antibody for WB next. I am looking for examining pancreatic islet as well as testis in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-10-17

Answer

I viewed the website and datasheets of our anti-Islet 1 Rabbit Monoclonal antibody and it appears that M02969 has been validated on mouse in both IP and WB. Thus M02969 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-17

Question

Can you help my question with product M02969, anti-Islet 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Moore

Verified customer

Asked: 2018-06-21

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02969 anti-Islet 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-21

Question

Will M02969 anti-Islet 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Carter

Verified customer

Asked: 2016-10-26

Answer

As indicated on the product datasheet, M02969 anti-Islet 1 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-10-26

Question

Our lab want to know about using your anti-Islet 1 Rabbit Monoclonal antibody for positive regulation of interleukin-1 alpha production studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.

N. Taylor

Verified customer

Asked: 2015-11-03

Answer

I appreciate your inquiry. This M02969 anti-Islet 1 Rabbit Monoclonal antibody is validated on k562 cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-11-03

Question

We are currently using anti-Islet 1 Rabbit Monoclonal antibody M02969 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?

E. Moore

Verified customer

Asked: 2014-11-26

Answer

The anti-Islet 1 Rabbit Monoclonal antibody (M02969) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-11-26

Question

Is a blocking peptide available for product anti-Islet 1 Rabbit Monoclonal antibody (M02969)?

T. Jackson

Verified customer

Asked: 2014-06-13

Answer

We do provide the blocking peptide for product anti-Islet 1 Rabbit Monoclonal antibody (M02969). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-06-13

Question

Here is the WB image, lot number and protocol we used for pancreatic islet using anti-Islet 1 Rabbit Monoclonal antibody M02969. Please let me know if you require anything else.

M. Walker

Verified customer

Asked: 2013-09-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-09-11

Question

We have seen staining in human testis. Are there any suggestions? Is anti-Islet 1 Rabbit Monoclonal antibody supposed to stain testis positively?

O. Walker

Verified customer

Asked: 2013-02-25

Answer

According to literature testis does express ISL1. According to Uniprot.org, ISL1 is expressed in forebrain, pancreatic islet, testis, platelet, among other tissues. Regarding which tissues have ISL1 expression, here are a few articles citing expression in various tissues:
Pancreatic islet, Pubmed ID: 7912209
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-02-25

Order DetailsPrice
M02969

100μL

$370
M02969-30ul

30μL sample

$99
M02969-carrier-free

Carrier Free

$370

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M02969
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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