|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-JAB1 Antibody|
|Description||Rabbit IgG polyclonal antibody for COP9 signalosome complex subunit 5(COPS4) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-JAB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2265)|
|Replacement Item||This antibody may replace the following items: sc-13157|sc-13157-X|sc-135954|sc-393725|sc-393725-X|sc-6271|sc-6271-X|sc-9074|sc-9074-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human JAB1(318-334aa LMSQVIKDKLFNQINIS), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||COP9 signalosome complex subunit 5|
|Molecular Weight||37579 MW|
|Protein Function||Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. .|
|Sequence Similarities||Belongs to the peptidase M67A family. CSN5 subfamily.|
|Subcellular Localization||Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Nuclear localization is diminished in the presence of IFIT3.|
|Alternative Names||COP9 signalosome complex subunit 5;SGN5;Signalosome subunit 5;3.4.-.-;Jun activation domain-binding protein 1;COPS5;CSN5, JAB1;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|p53| neuroscience|neurotransmission|intracellular signaling|kinases| cell biology|proteolysis / ubiquitin|proteasome / ubiquitin|proteasome|ubiquitin e3 enzymes|scf complex e3 ligase|other e3 ligases| signal transduction|protein phosphorylation|ser / thr kinases|other kinases|regulation| epigenetics and nuclear signaling|transcription|cancer susceptibility|proto-oncogenes| cancer|p53 pathway|ubiquitin & ubiquitin like modifiers|deubiquitination||
Background for COP9 signalosome complex subunit 5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-JAB1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-JAB1 Antibody Images
Click the images to enlarge.
All lanes: Anti-COPS5(PA2265) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,