Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody

Boster Bio Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody catalog # A00027-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00027-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody

See all JAK2 products

SKU/Catalog Number

A00027-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody catalog # A00027-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00027-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human JAK2 around the non-phosphorylation site of Y570.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00027-1 is reactive to JAK2 in Human, Mouse, Rat

Applications

A00027-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For JAK2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O60674

Gene ID

3717

Gene Name

JAK2

Full Name

Tyrosine-protein kinase JAK2

Weight

130.674kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.10; EC 2.7.10.2; Jak2; JAK-2; Janus kinase 2Janus kinase 2 (a protein tyrosine kinase); JTK10; tyrosine-protein kinase JAK2 JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on JAK2, check out the JAK2 Infographic

JAK2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for JAK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00027-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

He J,Zhang W,Di T,Meng J,Qi Y,Li G,Zhang Y,Su H,Yan W.Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody.Biomed Pharmacother.2020
Species: Human,Mouse
A00027-1 usage in article: APP:WB, SAMPLE:HOS CELL AND K7M2 CELL, DILUTION:1:500

Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
A00027-1 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300

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Find answers in Q&As, reviews.

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14 Customer Q&As for Anti-Tyrosine-protein kinase JAK2 JAK2 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-JAK2 antibody A00027-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-21

Question

I was wanting to use your anti-JAK2 antibody for WB for mouse fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse fetal brain identification?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

You can see on the product datasheet, A00027-1 anti-JAK2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-13

Question

Is a blocking peptide available for product anti-JAK2 antibody (A00027-1)?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

We do provide the blocking peptide for product anti-JAK2 antibody (A00027-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-24

Question

We want to test anti-JAK2 antibody A00027-1 on mouse fetal brain for research purposes, then I may be interested in using anti-JAK2 antibody A00027-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Kulkarni

Verified customer

Asked: 2020-01-14

Answer

The products we sell, including anti-JAK2 antibody A00027-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-14

Question

Is this A00027-1 anti-JAK2 antibody reactive to the isotypes of JAK2?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

The immunogen of A00027-1 anti-JAK2 antibody is Synthesized peptide derived from human JAK2 around the non-phosphorylation site of Y570. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-27

Question

Will A00027-1 anti-JAK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

You can see on the product datasheet, A00027-1 anti-JAK2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-07

Question

We are currently using anti-JAK2 antibody A00027-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

The anti-JAK2 antibody (A00027-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-23

Question

See attached the WB image, lot number and protocol we used for fetal brain using anti-JAK2 antibody A00027-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-24

Question

My colleagues were content with the WB result of your anti-JAK2 antibody. However we have seen positive staining in erythroleukemia endomembrane system using this antibody. Is that expected? Could you tell me where is JAK2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

From literature, erythroleukemia does express JAK2. Generally JAK2 expresses in endomembrane system. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310

Boster Scientific Support

Answered: 2018-05-18

Question

Will anti-JAK2 antibody A00027-1 work for WB with fetal brain?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

According to the expression profile of fetal brain, JAK2 is highly expressed in fetal brain. So, it is likely that anti-JAK2 antibody A00027-1 will work for WB with fetal brain.

Boster Scientific Support

Answered: 2018-02-15

Question

Do you have a BSA free version of anti-JAK2 antibody A00027-1 available?

S. Huang

Verified customer

Asked: 2017-10-17

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-JAK2 antibody A00027-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-17

Question

Can you help my question with product A00027-1, anti-JAK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-13

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00027-1 anti-JAK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-13

Question

I see that the anti-JAK2 antibody A00027-1 works with WB, what is the protocol used to produce the result images on the product page?

J. Taylor

Verified customer

Asked: 2017-09-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-11

Question

We have been able to see staining in rat popliteal artery. What should we do? Is anti-JAK2 antibody supposed to stain popliteal artery positively?

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

From what I have seen in literature popliteal artery does express JAK2. From what I have seen in Uniprot.org, JAK2 is expressed in popliteal artery, fetal brain, erythroleukemia, among other tissues. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310

Boster Scientific Support

Answered: 2017-08-21

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A00027-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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