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SKU:PA1629
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-JAK2 Antibody
SKU/Catalog Number PA1629
Description Rabbit IgG polyclonal antibody for Tyrosine-protein kinase JAK2(JAK2) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-JAK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1629)
Replacement Item This antibody may replace the following items: sc-34480|sc-278|sc-294|sc-34479|sc-7229|sc-390539|sc-294-G|sc-34479-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human JAK2(1096-1113aa DEIYMIMTECWNNNVNQR).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name JAK2
Protein Name Tyrosine-protein kinase JAK2
Molecular Weight 130674 MW
Protein Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin. .
Tissue Specificity Ubiquitously expressed throughout most tissues. .
Sequence Similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Subcellular Localization Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .
Uniprot ID O60674
Alternative Names Tyrosine-protein kinase JAK2;2.7.10.2;Janus kinase 2;JAK-2;JAK2;
Research Areas |cell biology|cell cycle|kinases/phosphatases| cell biology|cell cycle inhibitors| signal transduction|protein phosphorylation|tyrosine kinases| epigenetics and nuclear signaling|nuclear signaling pathways|stats| cancer|oncoproteins/suppressors|oncoproteins|signal transducers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tyrosine-protein kinase JAK2

Janus kinase 2, commanly called JAK2, is a human protein that has been implicated in signaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors. Exactly, JAK2 kinase is a member of a family of tyrosine kinases involved in cytokine receptor signaling. The JAK2 gene is mapped to 9p24.1. The JAK2 gene encodes a 1,132-amino acid protein that shares 95% sequence similarity to rat and pig Jak2. JAK2 is constitutively associated with the prolactin receptor and that it is activated and tyrosine phosphorylated upon PRL binding to the PRL receptor. JAK2, and more specifically just its intact N-terminal domain, binds to EPOR in the endoplasmic reticulum and promotes its cell surface expression. The human JAK2 is present in the nucleus of hematopoietic cells and directly phosphorylates tyr41 on histone H3.

Anti-JAK2 Antibody Images

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Anti-JAK2 Antibody
Anti- JAK2 antibody, PA1629, Western blotting
All lanes: Anti JAK2 (PA1629) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
Anti-JAK2 Antibody
Anti- JAK2 antibody, PA1629, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-JAK2 Antibody
Anti- JAK2 antibody, PA1629, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to jak2 antibody, jak 2 antibody, jak-2 antibody