Product Info Summary
SKU: | A05609-2 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-JunD (Ab-255) Antibody
SKU/Catalog Number
A05609-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-JunD (Ab-255) Antibody (Catalog # A05609-2). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JunD (Ab-255) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05609-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Clone Number
1E3
Isotype
IgG
Immunogen
Peptide sequence around aa. 253~257 (G-E-S-P-P) derived from Human JunD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05609-2 is reactive to JUND in Human
Applications
A05609-2 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
35.174kDa
Background of JUND
Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of JUND using anti-JUND antibody (A05609-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUND antigen affinity purified polyclonal antibody (Catalog # A05609-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for JUND.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using JunD(Ab-255) Antibody #A05609-2.
Click image to see more details
Figure 2. IHC analysis of JUND using anti-JUND antibody (A05609-2).
JUND was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-JUND Antibody (A05609-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For JUND (Source: Uniprot.org, NCBI)
Gene Name
JUND
Full Name
Transcription factor jun-D
Weight
35.174kDa
Superfamily
bZIP family
Alternative Names
activator protein 1; AP-1; jun D proto-oncogene; JunD; JunD-FL isoform; transcription factor jun-D JUND AP-1 JunD proto-oncogene, AP-1 transcription factor subunit transcription factor jun-D|activator protein 1|jun D proto-oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JUND, check out the JUND Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JUND: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JunD (Ab-255) Antibody (A05609-2)
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No publications found for A05609-2
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