Anti-Kallikrein 4/KLK4 Antibody Picoband™

Kallikrein 4/Prostase/EMSP1 antibody

Boster Bio Anti-Kallikrein 4/KLK4 Antibody Picoband™ catalog # PB9260. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9260
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Kallikrein 4/KLK4 Antibody Picoband™

View all Kallikrein 4/Prostase/EMSP1 Antibodies

SKU/Catalog Number

PB9260

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Kallikrein 4/KLK4 Antibody Picoband™ catalog # PB9260. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Kallikrein 4/KLK4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9260)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Kallikrein 4 recombinant protein (Position: I31-S254).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9260 is reactive to KLK4 in Human

Applications

PB9260 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

27.032kDa

Background of Kallikrein 4/Prostase/EMSP1

Kallikrein-related peptidase 4, also known as PSTS or EMSP1, is a protein which in humans is encoded by the KLK4 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. In particular, they may serve as biomarkers for both prostate cancer and breast cancer. It has been found that KLK4 is a proliferative factor with effects on gene expression and it may have a role in prostate cancer development and progression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For KLK4 (Source: Uniprot.org, NCBI)

Gene Name

KLK4

Full Name

Kallikrein-4

Weight

27.032kDa

Superfamily

peptidase S1 family

Alternative Names

AI2A1; ARM1; EC 3.4.21; EC 3.4.21.-; EMSP; EMSP1; EMSP1MGC116827; Enamel matrix serine proteinase 1; kallikrein 4 (prostase, enamel matrix, prostate); Kallikrein 4; kallikrein-4; Kallikrein-like protein 1; kallikrein-related peptidase 4; KLK4; KLK-L1MGC116828; Prostase; PRSS17enamel matrix serine protease 1; PSTSandrogen-regulated message 1; Serine protease 17 KLK4 AI2A1, ARM1, EMSP, EMSP1, KLK-L1, PRSS17, PSTS, kallikrein kallikrein related peptidase 4 kallikrein-4|androgen-regulated message 1|enamel matrix serine protease 1|enamel matrix serine proteinase 1|kallikrein-like protein 1|prostase|serine protease 17

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KLK4, check out the KLK4 Infographic

KLK4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KLK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Kallikrein 4/KLK4 Antibody Picoband™

Question

We are currently using anti-Kallikrein 4/KLK4 antibody PB9260 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

The anti-Kallikrein 4/KLK4 antibody (PB9260) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-11

Question

Does anti-Kallikrein 4/KLK4 antibody PB9260 work for WB with prostatic carcinoma?

Verified Customer

Verified customer

Asked: 2019-02-04

Answer

According to the expression profile of prostatic carcinoma, KLK4 is highly expressed in prostatic carcinoma. So, it is likely that anti-Kallikrein 4/KLK4 antibody PB9260 will work for WB with prostatic carcinoma.

Boster Scientific Support

Answered: 2019-02-04

Question

Is this PB9260 anti-Kallikrein 4/KLK4 antibody reactive to the isotypes of KLK4?

P. Roberts

Verified customer

Asked: 2019-01-03

Answer

The immunogen of PB9260 anti-Kallikrein 4/KLK4 antibody is E.coli-derived human Kallikrein 4 recombinant protein (Position: I31-S254). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-03

Question

Would anti-Kallikrein 4/KLK4 antibody PB9260 work on goat WB with prostatic carcinoma?

Verified Customer

Verified customer

Asked: 2018-12-19

Answer

Our lab technicians have not validated anti-Kallikrein 4/KLK4 antibody PB9260 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Kallikrein 4/KLK4 antibody PB9260 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat prostatic carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-19

Question

I see that the anti-Kallikrein 4/KLK4 antibody PB9260 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-19

Question

Please see the WB image, lot number and protocol we used for prostatic carcinoma using anti-Kallikrein 4/KLK4 antibody PB9260. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-09

Question

Is a blocking peptide available for product anti-Kallikrein 4/KLK4 antibody (PB9260)?

D. Kulkarni

Verified customer

Asked: 2014-07-24

Answer

We do provide the blocking peptide for product anti-Kallikrein 4/KLK4 antibody (PB9260). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-07-24

Order DetailsPrice
PB9260

100μg

$370
PB9260-10ug

10μg sample (liquid)

$99
PB9260-Biotin

100 μg Biotin conjugated

$570
PB9260-Cy3

100 μg Cy3 conjugated

$570
PB9260-Dylight488

100 μg Dylight488 conjugated

$570
PB9260-Dylight550

100 μg Dylight550 conjugated

$570
PB9260-Dylight594

100 μg Dylight594 conjugated

$570
PB9260-FITC

100 μg FITC conjugated

$570
PB9260-HRP

100 μg HRP conjugated

$570
PB9260-APC

100 μg APC conjugated

$670
PB9260-PE

100 μg PE conjugated

$670
PB9260-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9260
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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