Product Info Summary
SKU: | PB9260 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Kallikrein 4/KLK4 Antibody Picoband™
View all Kallikrein 4/Prostase/EMSP1 Antibodies
SKU/Catalog Number
PB9260
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 4/KLK4 Antibody Picoband™ catalog # PB9260. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 4/KLK4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9260)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Kallikrein 4 recombinant protein (Position: I31-S254).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9260 is reactive to KLK4 in Human
Applications
PB9260 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
27 kDa
Calculated molecular weight
27.032kDa
Background of Kallikrein 4/Prostase/EMSP1
Kallikrein-related peptidase 4, also known as PSTS or EMSP1, is a protein which in humans is encoded by the KLK4 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. In particular, they may serve as biomarkers for both prostate cancer and breast cancer. It has been found that KLK4 is a proliferative factor with effects on gene expression and it may have a role in prostate cancer development and progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Picoband antibody, PB, Western blotting
All lanes: Anti Kallikrein 4 (PB9260) at 0.5ug/ml
WB: Recombinant Human Kallikrein 4 Protein 0.5ng
Predicted bind size: 25KD
Observed bind size: 25KD
Click image to see more details
Anti-Picoband antibody, PB, Western blotting
All lanes: Anti Kallikrein 4 (PB9260) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
Protein Target Info & Infographic
Gene/Protein Information For KLK4 (Source: Uniprot.org, NCBI)
Gene Name
KLK4
Full Name
Kallikrein-4
Weight
27.032kDa
Superfamily
peptidase S1 family
Alternative Names
AI2A1; ARM1; EC 3.4.21; EC 3.4.21.-; EMSP; EMSP1; EMSP1MGC116827; Enamel matrix serine proteinase 1; kallikrein 4 (prostase, enamel matrix, prostate); Kallikrein 4; kallikrein-4; Kallikrein-like protein 1; kallikrein-related peptidase 4; KLK4; KLK-L1MGC116828; Prostase; PRSS17enamel matrix serine protease 1; PSTSandrogen-regulated message 1; Serine protease 17 KLK4 AI2A1, ARM1, EMSP, EMSP1, KLK-L1, PRSS17, PSTS, kallikrein kallikrein related peptidase 4 kallikrein-4|androgen-regulated message 1|enamel matrix serine protease 1|enamel matrix serine proteinase 1|kallikrein-like protein 1|prostase|serine protease 17
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK4, check out the KLK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 4/KLK4 Antibody Picoband™ (PB9260)
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No publications found for PB9260
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Kallikrein 4/KLK4 Antibody Picoband™
Question
We are currently using anti-Kallikrein 4/KLK4 antibody PB9260 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-Kallikrein 4/KLK4 antibody (PB9260) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
Does anti-Kallikrein 4/KLK4 antibody PB9260 work for WB with prostatic carcinoma?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
According to the expression profile of prostatic carcinoma, KLK4 is highly expressed in prostatic carcinoma. So, it is likely that anti-Kallikrein 4/KLK4 antibody PB9260 will work for WB with prostatic carcinoma.
Boster Scientific Support
Answered: 2019-02-04
Question
Is this PB9260 anti-Kallikrein 4/KLK4 antibody reactive to the isotypes of KLK4?
P. Roberts
Verified customer
Asked: 2019-01-03
Answer
The immunogen of PB9260 anti-Kallikrein 4/KLK4 antibody is E.coli-derived human Kallikrein 4 recombinant protein (Position: I31-S254). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-03
Question
Would anti-Kallikrein 4/KLK4 antibody PB9260 work on goat WB with prostatic carcinoma?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
Our lab technicians have not validated anti-Kallikrein 4/KLK4 antibody PB9260 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Kallikrein 4/KLK4 antibody PB9260 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat prostatic carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-19
Question
I see that the anti-Kallikrein 4/KLK4 antibody PB9260 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-19
Question
Please see the WB image, lot number and protocol we used for prostatic carcinoma using anti-Kallikrein 4/KLK4 antibody PB9260. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-09
Question
Is a blocking peptide available for product anti-Kallikrein 4/KLK4 antibody (PB9260)?
D. Kulkarni
Verified customer
Asked: 2014-07-24
Answer
We do provide the blocking peptide for product anti-Kallikrein 4/KLK4 antibody (PB9260). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-24