Anti-KDEL Monoclonal Antibody (10C3)


SKU M00955-2
Size 1mg
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number 10C3
Host Mouse
Applications ELISA, IP, IF, IHC, WB

Overview

Product Name Anti-KDEL Monoclonal Antibody (10C3)
SKU/Catalog Number M00955-2
Storage & Handling -20°C
Size 1mg
Description Mouse Monoclonal 10C3 antibody for Hspa5 {ECO:0000312 detection. Tested positive for ELISA, Flow Cytometry, IF, IF, IHC, IP, WB in -, -, Human|Mouse|Rat, -, -
Cite This Product Anti-KDEL Monoclonal Antibody (10C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955-2)
Host Mouse
Contents/Buffer Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Form Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Immunogen Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Images And Assay Conditions

Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).

Figure 1. IHC analysis of Hspa5 using anti-Hspa5 antibody (M00955-2).
Hspa5 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Hspa5 Antibody (M00955-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. Western blot analysis of Hspa5 using anti-Hspa5 antibody (M00955-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hspa5 antigen affinity purified polyclonal antibody (Catalog # M00955-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Hspa5.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P06761
Gene Name Hspa5

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.

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Mouse Monoclonal 10C3 antibody for Hspa5 {ECO:0000312 detection. Tested positive for ELISA, Flow Cytometry, IF, IF, IHC, IP, WB in -, -, Human|Mouse|Rat, -, -
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In stock
Order Product
M00955-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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Publications

Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways
Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.
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