Anti-KDEL Monoclonal Antibody (10C3)

GRP78/HSPA5 antibody

Boster Bio Anti-KDEL Monoclonal Antibody (10C3) catalog # M00955-2. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: M00955-2
Size: 50 µg
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: ELISA, IP, IF, IHC, WB

Customers Who Bought This Also Bought

Product Name

Anti-KDEL Monoclonal Antibody (10C3)

View all GRP78/HSPA5 Antibodies

SKU/Catalog Number

M00955-2

Size

50 µg

Form

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Description

Boster Bio Anti-KDEL Monoclonal Antibody (10C3) catalog # M00955-2. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KDEL Monoclonal Antibody (10C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00955-2)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

10C3

Isotype

IgG

Immunogen

Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00955-2 is reactive to HSPA5 in Human, Mouse, Rat

Applications

M00955-2 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

72.333kDa

Background of GRP78/HSPA5

Anti Apolipoprotein C-III antibody recognizes the gene product of APOC. Apolipoprotein C-III is a protein component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. This antibody is suitable for cardiovascular research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For HSPA5 (Source: Uniprot.org, NCBI)

Gene Name

HSPA5

Full Name

Endoplasmic reticulum chaperone BiP

Weight

72.333kDa

Superfamily

heat shock protein 70 family

Alternative Names

78 kDa glucose-regulated protein; BIP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; FLJ26106; GRP78; GRP-78; GRP78BIP; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSP70-5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2 HSPA5 BIP, GRP78, HEL-S-89n heat shock protein family A (Hsp70) member 5 endoplasmic reticulum chaperone BiP|78 kDa glucose-regulated protein|HSP70 family protein 5|binding-immunoglobulin protein|endoplasmic reticulum lumenal Ca(2+)-binding protein grp78|epididymis secretory sperm binding protein Li 89n|glucose-regulated protein, 78kDa|heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|heat shock protein 70 family protein 5|heat shock protein family A member 5|immunoglobulin heavy chain-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPA5, check out the HSPA5 Infographic

HSPA5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00955-2 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
M00955-2 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1200

Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways

Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning

Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...

Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.

Have you used Anti-KDEL Monoclonal Antibody (10C3) ?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-KDEL Monoclonal Antibody (10C3)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-KDEL Monoclonal Antibody (10C3)

Question

We ordered your anti-KDEL Monoclonal antibody (10C3) for IF on mammary carcinoma in the past. I am using human, and We are going to use the antibody for IHC next. I am interested in examining mammary carcinoma as well as colon carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

I have checked the website and datasheets of our anti-KDEL Monoclonal antibody (10C3) and it appears that M00955-2 has been validated on human in both IF and IHC. Thus M00955-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-10

Question

We have seen staining in mouse liver. Do you have any suggestions? Is anti-KDEL Monoclonal antibody (10C3) supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

Based on literature liver does express HSPA5. Based on Uniprot.org, HSPA5 is expressed in vena cava, cervix carcinoma, fibroblast, muscle, articular cartilage, mammary carcinoma, colon carcinoma, brain, cajal-retzius cell fetal brain cortex, melanoma, liver, among other tissues. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-27

Question

My colleagues were content with the WB result of your anti-KDEL Monoclonal antibody (10C3) . However we have seen positive staining in fibroblast endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is HSPA5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-10-18

Answer

From literature, fibroblast does express HSPA5. Generally HSPA5 expresses in endoplasmic reticulum lumen. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-10-18

Question

We are currently using anti-KDEL Monoclonal antibody (10C3) M00955-2 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2017-12-07

Answer

The anti-KDEL Monoclonal antibody (10C3) (M00955-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-07

Order DetailsPrice
M00955-2

1mg

$323

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00955-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$323.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.