Show Order Info
SKU:PB9026
Clonality:Polyclonal
Application:IHC-P, ICC, WB
Price: $240.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-Ki67 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Antigen KI-67(MKI67) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-Ki67 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9026)
Replacement Item This antibody may replace the following items: sc-101861|sc-107819|sc-15402|sc-23900|sc-292761|sc-52904|sc-56319|sc-7844|sc-7844-R|sc-7846 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MKI67
Protein Name Antigen KI-67
Molecular Weight 358694 MW
Protein Function Thought to be required for maintaining cell proliferation.
Sequence Similarities Contains 1 FHA domain.
Subcellular Localization Nucleus . Nucleus, nucleolus . Chromosome . Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
Uniprot ID P46013
Alternative Names Antigen KI-67;MKI67;
Research Areas |cell biology|cell cycle|markers| neuroscience|cell type marker|neuron marker|soma marker| tags & cell markers|cell type markers|replication|neurology process|neurogenesis| cancer|cell division|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Antigen KI-67

Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Anti-Ki67 Picoband™ Antibody Images

Click the images to enlarge.

Anti-Ki67 Picoband™ Antibody
Anti-Ki67 Picoband antibody, PB9026-1.JPG
IHC(P): Human Tonsil Tissue
Anti-Ki67 Picoband™ Antibody
Anti-Ki67 Picoband antibody, PB9026-2.jpg
All lanes: Anti-Ki67(PB9026) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 358KD
Observed bind size: 358KD
Write a review for PB9026

Publications

Cai Mj, Xie Rf, Han L, Chen Rd, Wang Bf, Ye F, Guo Ds, Lei T. Ai Zheng. 2009 Jan;28(1):1-4. Epub 2009 Jan 28. Effect Of Rnai-Mediated Lrig3 Gene Silencing On Proliferation Of Glioma Gl15 Cells And Expression Of Pcna And Ki-67.
Qiu M, Bao W, Wang J, Yang T, He X, Liao Y, Wan X. Bmc Cancer. 2014 Feb 11;14:78. Doi: 10.1186/1471-2407-14-78. Foxa1 Promotes Tumor Cell Proliferation Through Ar Involving The Notch Pathway In Endometrial Cancer.
Wang Y, Qiu H, Hu W, Li S, Yu J. Oncol Rep. 2014 Mar;31(3):1389-95. Doi: 10.3892/Or.2014.2990. Epub 2014 Jan 21. Rprd1B Promotes Tumor Growth By Accelerating The Cell Cycle In Endometrial Cancer.
Fang Lq, Zhang H, Ding Xy, Li Dq, Hou Xl, Qiao H, Bai J, Wang Zb. Cancer Lett. 2010 Dec 28;299(2):111-8. Doi: 10.1016/J.Canlet.2010.08.008. Epub 2010 Sep 9. Mouse Trophoblastic Cells Exhibit A Dominant Invasiveness Phenotype Over Cancer Cells.
Zhao Xy, Chen L, Li Yh, Xu Q. World J Gastroenterol. 2007 Dec 28;13(48):6558-61. Plexina1 Expression In Gastric Carcinoma And Its Relationship With Tumor Angiogenesis And Proliferation.
Zhao W, Han Q, Lin H, Sun W, Gao Y, Zhao Y, Wang B, Wang X, Chen B, Xiao Z, Dai J. Tissue Eng Part A. 2009 May;15(5):991-8. Doi: 10.1089/Ten.Tea.2008.0240. Human Basic Fibroblast Growth Factor Fused With Kringle4 Peptide Binds To A Fibrin Scaffold...
Song Lj, Liu Rj, Zeng Z, Alper Sl, Cui Hj, Lu Y, Zheng L, Yan Zw, Fu Gh. J Mol Med (Berl). 2012 Jun;90(6):707-18. Doi: 10.1007/S00109-011-0851-2. Epub 2012 Jan 7. Gastrin Inhibits A Novel, Pathological Colon Cancer Signaling Pathway Involving Egr1...
Li Dw, Sun Yj, Sun Zf, Dong P. J Laryngol Otol. 2012 Nov;126(11):1127-33. Doi: 10.1017/S0022215112001971. Epub 2012 Sep 11. Involvement Of Focal Adhesion Kinase In Cellular Proliferation, Apoptosis And Prognosis Of Laryngeal Squamous Cell Carcinoma.
Yuan W, Xiaoyun H, Haifeng Q, Jing L, Weixu H, Ruofan D, Jinjin Y, Zongji S. Int J Med Sci. 2014 May 6;11(7):691-6. Doi: 10.7150/Ijms.8880. Ecollection 2014. Microrna-218 Enhances The Radiosensitivity Of Human Cervical Cancer Via Promoting Radiati...
Dong R, Qiu H, Du G, Wang Y, Yu J, Mao C. Mol Med Rep. 2014 Dec;10(6):3289-95. Doi: 10.3892/Mmr.2014.2622. Epub 2014 Oct 10. Restoration Of Microrna
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Feng D, Cao Z, Li C, Zhang L, Zhou Y, Ma J, Liu R, Zhou H, Zhao W, Wei H, Ling B. Curr Mol Med. 2012 Mar;12(3):342-54. Combination Of Valproic Acid And Atra Restores Rar??2 Expression And Induces Differentiation In Cervical Cancer Through The Pi3K/...
Huang C, Ma R, Xu Y, Li N, Li Z, Yue J, Li H, Guo Y, Qi D. Am J Cancer Res. 2015 Feb 15;5(3):1032-46. Ecollection 2015. Wnt2 Promotes Non-Small Cell Lung Cancer Progression By Activating Wnt/??-Catenin Pathway.
Liu W, Wang Jc, Wang J. Lab Chip. 2015 Feb 21;15(4):1195-204. Doi: 10.1039/C4Lc01242A. Controllable Organization And High Throughput Production Of Recoverable 3D Tumors Using Pneumatic Microfluidics.
Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.
Yang Tq, Lu Xj, Wu Tf, Ding Dd, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Guo Lc, Zhang Y, Huang Yl, Zhou Yx, Du Zw. Cancer Sci. 2014 Mar;105(3):265-71. Doi: 10.1111/Cas.12351. Epub 2014 Feb 11. Microrna-16 Inhibits Glioma Cell Growth And Invasion T...
Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...
Wang Q, Zhong Yj, Yuan Jp, Shao Lh, Zhang J, Tang L, Liu Sp, Hong Yp, Firestone Ra, Li Y. J Transl Med. 2013 Aug 21;11:192. Doi: 10.1186/1479-5876-11-192. Targeting Therapy Of Hepatocellular Carcinoma With Doxorubicin Prodrug Pdox Increases Anti-M...
Shan S, Li Z, Newton Ip, Zhao C, Li Z, Guo M. Toxicol Lett. 2014 Jun 5;227(2):129-38. Doi: 10.1016/J.Toxlet.2014.03.008. Epub 2014 Mar 28. A Novel Protein Extracted From Foxtail Millet Bran Displays Anti-Carcinogenic Effects In Human Colon Cancer ...
Alsaegh Ma, Miyashita H, Zhu Sr. Pathol Oncol Res. 2015 Jan;21(1):65-71. Doi: 10.1007/S12253-014-9789-3. Epub 2014 May 15. Expression Of Human Papillomavirus Is Correlated With Ki-67 And Cox-2 Expressions In Keratocystic Odontogenic Tumor.
Chang L, Guo F, Lv Y, Wang Y, Huo B, Wang L, Liu W. Mol Biol Rep. 2013 Jan;40(1):669-73. Doi: 10.1007/S11033-012-2106-X. Epub 2012 Oct 11. The Inhibitory Effects Of Endostar Combined With Chemotherapy On Human Esophageal Squamous Cell Carcinoma Xe...
Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
Yi M, Yang J, Chen X, Li J, Li X, Wang L, Tan Y, Xiong W, Zhou M, Mccarthy Jb, Li G, Xiang B, Xie H. J Cell Physiol. 2011 Sep;226(9):2360-9. Doi: 10.1002/Jcp.22568. Rassf1A Suppresses Melanoma Development By Modulating Apoptosis And Cell-Cycle Pro...
Bao W, Fan Q, Luo X, Cheng Ww, Wang Yd, Li Zn, Chen Xl, Wu D. Oncol Rep. 2013 Aug;30(2):723-30. Doi: 10.3892/Or.2013.2496. Epub 2013 May 27. Silencing Of Cathepsin B Suppresses The Proliferation And Invasion Of Endometrial Cancer.
Chen G, Liang Yx, Zhu Jg, Fu X, Chen Yf, Mo Rj, Zhou L, Fu H, Bi Xc, He Hc, Yang Sb, Wu Yd, Jiang Fn, Zhong Wd. Biomed Res Int. 2014;2014:230183. Doi: 10.1155/2014/230183. Epub 2014 Aug 17. Cc Chemokine Ligand 18 Correlates With Malignant Progress...
Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.