Anti-Ki67 MKI67 Rabbit Monoclonal Antibody

Ki67/MKI67 antibody

Boster Bio Anti-Ki67 MKI67 Rabbit Monoclonal Antibody catalog # M00254. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: M00254
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Ki67 MKI67 Rabbit Monoclonal Antibody

View all Ki67/MKI67 Antibodies

SKU/Catalog Number

M00254

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Ki67 MKI67 Rabbit Monoclonal Antibody catalog # M00254. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ki67 MKI67 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EAH-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Ki67

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00254 is reactive to MKI67 in Human

Applications

M00254 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

358.694kDa

Background of Ki67/MKI67

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)

Gene Name

MKI67

Full Name

Proliferation marker protein Ki-67

Weight

358.694kDa

Alternative Names

antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; antiKi67; Ki67 ihc; Ki-67 ihc; Ki67 mouse; Ki-67 mouse; Ki67 western blot; Ki-67 western blot; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67|antigen Ki67|antigen identified by monoclonal antibody Ki-67|proliferation-related Ki-67 antigen|protein phosphatase 1, regulatory subunit 105

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MKI67, check out the MKI67 Infographic

MKI67 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00254 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
M00254 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA

KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1

715: GNRs@SiO2-FA in combination with radiotherapy induces the apoptosis of HepG2 cells by modulating the expression of apoptosis-related proteins

Mutant p53 induces EZH2 expression and promotes epithelial?mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing

Solamargine triggers hepatoma cell death through apoptosis

WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects

Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo

MicroRNA-150 suppresses cell proliferation and metastasis in hepatocellular carcinoma by inhibiting the GAB1-ERK axis

Soy isoflavone extracts stimulate the growth of nude mouse xenografts bearing estrogen-dependent human breast cancer cells (MCF-7)

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7 Customer Q&As for Anti-Ki67 MKI67 Rabbit Monoclonal Antibody

Question

Is this M00254 anti-Ki67 Rabbit Monoclonal antibody reactive to the isotypes of MKI67?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

The immunogen of M00254 anti-Ki67 Rabbit Monoclonal antibody is A synthesized peptide derived from human Ki67. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-06

Question

Does M00254 anti-Ki67 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-08-20

Answer

As indicated on the product datasheet, M00254 anti-Ki67 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-20

Question

Does anti-Ki67 Rabbit Monoclonal antibody M00254 work for IHC with lung?

Verified Customer

Verified customer

Asked: 2018-05-31

Answer

According to the expression profile of lung, MKI67 is highly expressed in lung. So, it is likely that anti-Ki67 Rabbit Monoclonal antibody M00254 will work for IHC with lung.

Boster Scientific Support

Answered: 2018-05-31

Question

I see that the anti-Ki67 Rabbit Monoclonal antibody M00254 works with IHC, what is the protocol used to produce the result images on the product page?

J. Li

Verified customer

Asked: 2016-12-08

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-12-08

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Ki67 Rabbit Monoclonal antibody M00254. Let me know if you need anything else.

L. Brown

Verified customer

Asked: 2015-12-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-22

Question

See attached the WB image, lot number and protocol we used for lung using anti-Ki67 Rabbit Monoclonal antibody M00254. Please let me know if you require anything else.

J. Lewis

Verified customer

Asked: 2014-08-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-08-05

Question

I am interested in to test anti-Ki67 Rabbit Monoclonal antibody M00254 on human lung for research purposes, then I may be interested in using anti-Ki67 Rabbit Monoclonal antibody M00254 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Kulkarni

Verified customer

Asked: 2014-06-30

Answer

The products we sell, including anti-Ki67 Rabbit Monoclonal antibody M00254, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-06-30

Order DetailsPrice
M00254

100μL

$370
M00254-30ul

30μL sample

$99
M00254-carrier-free

Carrier Free

$370

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M00254
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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