Anti-KMT6/EZH2 Antibody Picoband™

EZH2/KMT6 antibody

Boster Bio Anti-KMT6/EZH2 Antibody Picoband™ catalog # A00050-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00050-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-KMT6/EZH2 Antibody Picoband™

View all EZH2/KMT6 Antibodies

SKU/Catalog Number

A00050-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KMT6/EZH2 Antibody Picoband™ catalog # A00050-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KMT6/EZH2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00050-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KMT6/EZH2 recombinant protein (Position: E22-T345).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00050-1 is reactive to EZH2 in Human, Mouse, Rat

Applications

A00050-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

98 kDa

Calculated molecular weight

85.363kDa

Background of EZH2/KMT6

Enhancer of zeste homolog 2 (EZH2) is a histone-lysine N-methyltransferase enzyme encoded by EZH2 gene. It is mapped to 7q36.1. This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For EZH2 (Source: Uniprot.org, NCBI)

Gene Name

EZH2

Full Name

Histone-lysine N-methyltransferase EZH2

Weight

85.363kDa

Superfamily

class V-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1; EC 2.1.1.43; enhancer of zeste (Drosophila) homolog 2; enhancer of zeste 2; enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste homolog 2; ENX1; ENX-1KMT6EZH1KMT6A; EZH2; histone-lysine N-methyltransferase EZH2; KMT6; Lysine N-methyltransferase 6; MGC9169 EZH2 ENX-1, ENX1b, KMT6, KMT6A, WVS, WVS2, EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit histone-lysine N-methyltransferase EZH2|enhancer of zeste homolog 2|lysine N-methyltransferase 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EZH2, check out the EZH2 Infographic

EZH2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EZH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00050-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Enhancer of zeste homolog 2 silencing inhibits tumor growth and lung metastasis in osteosarcoma

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16 Customer Q&As for Anti-KMT6/EZH2 Antibody Picoband™

Question

I am looking for using your anti-KMT6/EZH2 antibody for skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

We appreciate your inquiry. This A00050-1 anti-KMT6/EZH2 antibody is validated on human k562, k562 whole cell lysates, mouse sp20 whole cell lysates. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-06

Question

I was wanting to use your anti-KMT6/EZH2 antibody for ELISA for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

It shows on the product datasheet, A00050-1 anti-KMT6/EZH2 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-KMT6/EZH2 antibody A00050-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-23

Question

I see that the anti-KMT6/EZH2 antibody A00050-1 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-10

Question

We have observed staining in rat cervix carcinoma. What should we do? Is anti-KMT6/EZH2 antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

From what I have seen in literature cervix carcinoma does express EZH2. From what I have seen in Uniprot.org, EZH2 is expressed in lung, brain, brain testis, pancreas, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-10

Question

Is a blocking peptide available for product anti-KMT6/EZH2 antibody (A00050-1)?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

We do provide the blocking peptide for product anti-KMT6/EZH2 antibody (A00050-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-10

Question

We ordered your anti-KMT6/EZH2 antibody for ELISA on lung last year. I am using human, and We want to use the antibody for WB next. I am interested in examining lung as well as brain testis in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-04-24

Answer

I took a look at the website and datasheets of our anti-KMT6/EZH2 antibody and it appears that A00050-1 has been validated on human in both ELISA and WB. Thus A00050-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-04-24

Question

Is this A00050-1 anti-KMT6/EZH2 antibody reactive to the isotypes of EZH2?

R. Patel

Verified customer

Asked: 2018-04-10

Answer

The immunogen of A00050-1 anti-KMT6/EZH2 antibody is E.coli-derived human KMT6/EZH2 recombinant protein (Position: E22-T345). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-04-10

Question

Will anti-KMT6/EZH2 antibody A00050-1 work for ELISA with lung?

Verified Customer

Verified customer

Asked: 2018-03-06

Answer

According to the expression profile of lung, EZH2 is highly expressed in lung. So, it is likely that anti-KMT6/EZH2 antibody A00050-1 will work for ELISA with lung.

Boster Scientific Support

Answered: 2018-03-06

Question

See attached the WB image, lot number and protocol we used for lung using anti-KMT6/EZH2 antibody A00050-1. Please let me know if you require anything else.

M. Bhatt

Verified customer

Asked: 2018-01-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-01

Question

My question regarding product A00050-1, anti-KMT6/EZH2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Parker

Verified customer

Asked: 2017-09-08

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00050-1 anti-KMT6/EZH2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-08

Question

My boss were satisfied with the WB result of your anti-KMT6/EZH2 antibody. However we have seen positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is EZH2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-17

Answer

Based on literature, cervix carcinoma erythroleukemia does express EZH2. Generally EZH2 expresses in nucleus. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-07-17

Question

Would A00050-1 anti-KMT6/EZH2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Evans

Verified customer

Asked: 2016-09-14

Answer

As indicated on the product datasheet, A00050-1 anti-KMT6/EZH2 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-09-14

Question

Is there a BSA free version of anti-KMT6/EZH2 antibody A00050-1 available?

A. Huang

Verified customer

Asked: 2016-06-01

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-KMT6/EZH2 antibody A00050-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-06-01

Question

We are currently using anti-KMT6/EZH2 antibody A00050-1 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

F. Moore

Verified customer

Asked: 2014-05-20

Answer

The anti-KMT6/EZH2 antibody (A00050-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-05-20

Question

you antibody to test anti-KMT6/EZH2 antibody A00050-1 on mouse lung for research purposes, then I may be interested in using anti-KMT6/EZH2 antibody A00050-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

W. Kulkarni

Verified customer

Asked: 2013-05-28

Answer

The products we sell, including anti-KMT6/EZH2 antibody A00050-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-05-28

Order DetailsPrice
A00050-1

100μg

$370
A00050-1-10ug

10μg sample (liquid)

$99
A00050-1-Biotin

100 μg Biotin conjugated

$570
A00050-1-Cy3

100 μg Cy3 conjugated

$570
A00050-1-Dylight488

100 μg Dylight488 conjugated

$570
A00050-1-Dylight550

100 μg Dylight550 conjugated

$570
A00050-1-Dylight594

100 μg Dylight594 conjugated

$570
A00050-1-FITC

100 μg FITC conjugated

$570
A00050-1-HRP

100 μg HRP conjugated

$570
A00050-1-APC

100 μg APC conjugated

$670
A00050-1-PE

100 μg PE conjugated

$670
A00050-1-carrier-free

Carrier Free

$370

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