Anti-KPNA2 Monoclonal Antibody

Importin alpha 2/KPNA2 antibody

Boster Bio Anti-KPNA2 Monoclonal Antibody catalog # M01776. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01776
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-KPNA2 Monoclonal Antibody

View all Importin alpha 2/KPNA2 Antibodies

SKU/Catalog Number

M01776

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-KPNA2 Monoclonal Antibody catalog # M01776. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KPNA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01776)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEAF-11

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human KPNA2 Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01776 is reactive to KPNA2 in Human, Mouse, Rat

Applications

M01776 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

57.862kDa

Background of Importin alpha 2/KPNA2

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For KPNA2 (Source: Uniprot.org, NCBI)

Gene Name

KPNA2

Full Name

Importin subunit alpha-1

Weight

57.862kDa

Superfamily

importin alpha family

Alternative Names

Importin alpha 1; Importin alpha 2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); Karyopherin subunit alpha-2; KPNA2; Pendulin; QIP2; QIP2importin alpha 2; RAG cohort 1; RAG cohort protein 1; RCH1; RCH1importin subunit alpha-2; SRP1; SRP1alpha; SRP1-alpha KPNA2 IPOA1, QIP2, RCH1, SRP1-alpha, SRP1alpha karyopherin subunit alpha 2 importin subunit alpha-1|RAG cohort protein 1|importin subunit alpha-2|importin-alpha-P1|karyopherin alpha 2 (RAG cohort 1, importin alpha 1)|pendulin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KPNA2, check out the KPNA2 Infographic

KPNA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KPNA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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8 Customer Q&As for Anti-KPNA2 Monoclonal Antibody

Question

What sample was tested in WB and IHC applications with M01776?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of KPNA2 expression in (1) HeLa cell lysate; (2) C6 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow testis using anti-KPNA2 Monoclonal antibody M01776. Let me know if you need anything else.

T. Brown

Verified customer

Asked: 2019-11-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-12

Question

See attached the WB image, lot number and protocol we used for bone marrow testis using anti-KPNA2 Monoclonal antibody M01776. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-01-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-04

Question

I see that the anti-KPNA2 Monoclonal antibody M01776 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-04-20

Question

My lab would like to test anti-KPNA2 Monoclonal antibody M01776 on human bone marrow testis for research purposes, then I may be interested in using anti-KPNA2 Monoclonal antibody M01776 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Miller

Verified customer

Asked: 2016-08-03

Answer

The products we sell, including anti-KPNA2 Monoclonal antibody M01776, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-08-03

Question

Is there a BSA free version of anti-KPNA2 Monoclonal antibody M01776 available?

B. Miller

Verified customer

Asked: 2016-05-03

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KPNA2 Monoclonal antibody M01776 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-05-03

Question

Is a blocking peptide available for product anti-KPNA2 Monoclonal antibody (M01776)?

G. Parker

Verified customer

Asked: 2015-04-28

Answer

We do provide the blocking peptide for product anti-KPNA2 Monoclonal antibody (M01776). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-04-28

Question

Does anti-KPNA2 Monoclonal antibody M01776 work for WB with bone marrow testis?

N. Carter

Verified customer

Asked: 2014-03-04

Answer

According to the expression profile of bone marrow testis, KPNA2 is highly expressed in bone marrow testis. So, it is likely that anti-KPNA2 Monoclonal antibody M01776 will work for WB with bone marrow testis.

Boster Scientific Support

Answered: 2014-03-04

Order DetailsPrice
M01776

100μL

$370
M01776-30ul

30μL sample

$99
M01776-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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