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SKU:PA1103
Clonality:Polyclonal
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Lamin A/C Antibody
Description Rabbit IgG polyclonal antibody for Prelamin-A/C(LMNA) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Cite This Product Anti-Lamin A/C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1103)
Replacement Item This antibody may replace the following items: sc-20680|sc-20681|sc-242223|sc-242224|sc-25104|sc-25105|sc-376248|sc-398927|sc-56137|sc-56140|sc-56141|sc-6214|sc-6215|sc-71481|sc-7292|sc-7292-X|sc-7293|sc-81611 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C(455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LMNA
Protein Name Prelamin-A/C
Molecular Weight 74139 MW
Protein Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone.
Tissue Specificity In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress. .
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin- A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C.
Uniprot ID P02545
Alternative Names Prelamin-A/C;Lamin-A/C;70 kDa lamin;Renal carcinoma antigen NY-REN-32;LMNA;LMN1;
Research Areas |tags & cell markers|subcellular markers|nucleus|nuclear envelope| signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class v|lamins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Prelamin-A/C

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C(LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Anti-Lamin A/C Antibody Images

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Anti-Lamin A/C Antibody
Anti-Lamin A+C antibody, PA1103, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: A431 Cell Lysate
Anti-Lamin A/C Antibody
Anti-Lamin A+C antibody, PA1103, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-Lamin A/C Antibody
Anti-Lamin A+C antibody, PA1103, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti-Lamin A/C Antibody
Anti-Lamin A+C antibody, PA1103, IHC(P)
IHC(P): Rat Intestine Tissue
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Publications

Yang Lm, Liu Xl, Zhu Qt, Zhang Y, Xi Tf, Hu J, He Cf, Jiang L. J Biomed Mater Res B Appl Biomater. 2011 Jan;96(1):25-33. Doi: 10.1002/Jbm.B.31719. Human Peripheral Nerve-Derived Scaffold For Tissue-Engineered Nerve Grafts: Histology And Biocompati...
Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lamin a/c antibody, lamin-a/c antibody, lamin a antibody, lamin antibody, lamin c antibody