Product Info Summary
SKU: | M00438-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Lamin A/C LMNA Monoclonal Antibody
SKU/Catalog Number
M00438-1
Size
100ul
Description
Boster Bio Anti-Lamin A/C LMNA Monoclonal Antibody catalog # M00438-1. Tested in IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lamin A/C LMNA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00438-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length recombinant human lamin A expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00438-1 is reactive to LMNA in Human
Applications
M00438-1 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
74.139kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000. IF/ICC 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of different cell lysates using mouse mAb to lamin A/C, M00438-1, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65kDa correspond to the lamin A and lamin C proteins respectively, detected only in the cells of human origin. M00438-1 antibody failed to recognize rat or mouse proteins.
Click image to see more details
Immunofluorescent analysis of HeLa cells stained with mouse mAb to lamin A/C, M00438-1, dilution 1:2,000 in red, and costained with rabbit pAb to HSP60, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. M00438-1 antibody specifically labels the nuclear lamina, while the other antibody reveals protein expressed in mitochondria.
Protein Target Info & Infographic
Gene/Protein Information For LMNA (Source: Uniprot.org, NCBI)
Gene Name
LMNA
Full Name
Prelamin-A/C
Weight
74.139kDa
Superfamily
intermediate filament family
Alternative Names
CDCD1; CDDC; CMD1A; CMT2B1; dilated 1A (autosomal dominant); EMD2; FPLD; HGPSFPL; lamin A/C; lamin A/C-like 1; lamin-A/C; LDP1; LFP; LGMD1B; limb girdle muscular dystrophy 1B (autosomal dominant); LMN1IDC; LMNC; LMNL1; prelamin-A/C; PRO1,70 kDa lamin; progeria 1 (Hutchinson-Gilford type); renal carcinoma antigen NY-REN-32 LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma antigen NY-REN-32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMNA, check out the LMNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lamin A/C LMNA Monoclonal Antibody (M00438-1)
Hello CJ!
No publications found for M00438-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Lamin A/C LMNA Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Lamin A/C LMNA Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Lamin A/C LMNA Monoclonal Antibody
Question
We have observed staining in human corpus callosum. Do you have any suggestions? Is anti-Lamin A/C LMNA Monoclonal antibody supposed to stain corpus callosum positively?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
From literature corpus callosum does express LMNA. From Uniprot.org, LMNA is expressed in mucosa of stomach, corpus callosum, kidney, lung skin, ovarian carcinoma, colon, renal cell carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2020-02-14
Question
Our lab were satisfied with the WB result of your anti-Lamin A/C LMNA Monoclonal antibody. However we have seen positive staining in corpus callosum isoform c: nucleus speckle using this antibody. Is that expected? Could you tell me where is LMNA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
From literature, corpus callosum does express LMNA. Generally LMNA expresses in nucleus., isoform c: nucleus speckle. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2019-12-16
Question
We bought anti-Lamin A/C LMNA Monoclonal antibody for WB on cervix carcinoma a few months ago. I am using human, and We want to use the antibody for IF next. We want examining cervix carcinoma as well as renal cell carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-03-13
Answer
I looked at the website and datasheets of our anti-Lamin A/C LMNA Monoclonal antibody and it seems that M00438-1 has been validated on human in both WB and IF. Thus M00438-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-13
Question
I am interested in using your anti-Lamin A/C LMNA Monoclonal antibody for regulation of protein localization to nucleus studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
G. Huang
Verified customer
Asked: 2018-04-05
Answer
We appreciate your inquiry. This M00438-1 anti-Lamin A/C LMNA Monoclonal antibody is validated on hela cells. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-05
Question
We are currently using anti-Lamin A/C LMNA Monoclonal antibody M00438-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
S. Patel
Verified customer
Asked: 2017-08-04
Answer
The anti-Lamin A/C LMNA Monoclonal antibody (M00438-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-04