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SKU:MA1054
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:LAM-89
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Laminin Antibody
SKU/Catalog Number MA1054
Description Mouse IgG monoclonal antibody for Laminin, laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2 (laminin S) (LAMA1; LAMA2; LAMB1; LAMB2) detection. Tested with WB, IHC-P in Human;feline;pig. No cross reactivity with other proteins.
Cite This Product Anti-Laminin Antibody LAM-89 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1054)
Replacement Item This antibody may replace the following items: sc-13144|sc-133241|sc-133722|sc-134477|sc-135967|sc-16581|sc-16582|sc-17751|sc-17763|sc-17810|sc-20142|sc-20777|sc-23410|sc-23411|sc-23412|sc-23414|sc-30267|sc-30268|sc-30974|sc-30975|sc-30976|sc-31094|sc-33708|sc-33709|sc-365214|sc-365962|sc-373918|sc-374015|sc-377379|sc-47766|sc-55605|sc-5582|sc-5583|sc-5584|sc-56146|sc-56147|sc-59846|sc-59849|sc-59854|sc-59980|sc-6016|sc-6018|sc-6019|sc-6216|sc-6217|sc-65578|sc-65643|sc-71484|sc-74417|sc-74418|sc-7650 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Feline, Human, Pig
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human laminin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number LAM-89
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LAMA1
Protein Name Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
Molecular Weight 196474 MW
Protein Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue Specificity Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
Sequence Similarities Contains 17 laminin EGF-like domains.
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Uniprot ID P15800
Alternative Names Laminin subunit beta-2;Laminin chain B3;Laminin-11 subunit beta;Laminin-14 subunit beta;Laminin-15 subunit beta;Laminin-3 subunit beta;Laminin-4 subunit beta;Laminin-7 subunit beta;Laminin-9 subunit beta;S-laminin subunit beta;S-LAM beta;Lamb2;
Research Areas cardiovascular|angiogenesis|adhesion / ecm|extracellular matrix|neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments|signal transduction|cytoskeleton / ecm|basal lamina|ecm proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2

Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2(LAMA2). This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.
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Publications

Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.
Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...
Chen W, Mo W, Sun K, Huang X, Zhang Yl, Song Hy. Curr Eye Res. 2009 Dec;34(12):1057-64. Doi: 10.3109/02713680903308487. Microplasmin Degrades Fibronectin And Laminin At Vitreoretinal Interface And Outer Retina During Enzymatic Vitrectomy.
Chen Tl, Wang Hl, Liu Yh, Fang Y, Tan Rz, Zhou Ph, Zhou Q, Lv Xy. Scientificworldjournal. 2014;2014:682189. Doi: 10.1155/2014/682189. Epub 2014 Jul 14. Highly Effective Ex Vivo Gene Manipulation To Study Kidney Development Using Self-Complementary...
Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.
He S, Xia T, Wang H, Wei L, Luo X, Li X. Acta Biomater. 2012 Jul;8(7):2659-69. Doi: 10.1016/J.Actbio.2012.03.044. Epub 2012 Apr 3. Multiple Release Of Polyplexes Of Plasmids Vegf And Bfgf From Electrospun Fibrous Scaffolds Towards Regeneration Of ...
Liu Y, Lu J, Li H, Wei J, Li X. Acta Biomater. 2015 Jan;11:114-25. Doi: 10.1016/J.Actbio.2014.10.004. Epub 2014 Oct 7. Engineering Blood Vessels Through Micropatterned Co-Culture Of Vascular Endothelial And Smooth Muscle Cells On Bilayered Electro...
Chen F, Wan H, Xia T, Guo X, Wang H, Liu Y, Li X. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):699-710. Doi: 10.1016/J.Ejpb.2013.07.009. Epub 2013 Jul 24. Promoted Regeneration Of Mature Blood Vessels By Electrospun Fibers With Loaded Multiple Pdna-C...
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lama antibody, laminin alpha 1 antibody