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SKU:PB9142
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, ICC, WB
Price: $240.00
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Overview

Product Name Anti-Laminin Picoband™ Antibody
SKU/Catalog Number PB9142
Description Rabbit IgG polyclonal antibody for Laminin subunit gamma-1&Laminin subunit gamma-2&Laminin subunit gamma-3(LAMC1&LAMC2&LAMC3) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-Laminin Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9142)
Replacement Item This antibody may replace the following items: sc-13144|sc-133241|sc-135967|sc-16601|sc-17751|sc-20776|sc-20777|sc-23412|sc-23414|sc-25341|sc-25719|sc-28330|sc-30974|sc-30975|sc-30976|sc-31087|sc-31092|sc-31094|sc-33708|sc-393225|sc-393502|sc-47766|sc-55457|sc-5584|sc-59846|sc-59980|sc-6019|sc-65578|sc-65643|sc-7650|sc-7652 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Peptide mixture of laminin gamma1,2,3(DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LAMC3
Protein Name Laminin subunit gamma-1&Laminin subunit gamma-2&Laminin subunit gamma-3
Molecular Weight 171227 MW
Protein Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue Specificity Broadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence Similarities Contains 11 laminin EGF-like domains.
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Uniprot ID Q9Y6N6
Alternative Names Laminin subunit gamma-3;Laminin-12 subunit gamma;Laminin-14 subunit gamma;Laminin-15 subunit gamma;LAMC3;
Research Areas cardiovascular|angiogenesis|adhesion / ecm|extracellular matrix|neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments|signal transduction|cytoskeleton / ecm|basal lamina|ecm proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Laminin subunit gamma-1&Laminin subunit gamma-2&Laminin subunit gamma-3

Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.

Anti-Laminin Picoband™ Antibody Images

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Anti-Laminin Picoband™ Antibody
Anti-Laminin Picoband antibody, PB9142-2.jpg
All lanes: Anti Laminin (PB9142) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 177KD
Observed bind size: 177KD
Anti-Laminin Picoband™ Antibody
Anti-Laminin Picoband antibody, PB9142-3.JPG
IHC(P): Human Lung Cancer Tissue
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Publications

Guo C, Xu L, He Q, Liang T, Duan X, Li R. Food Chem Toxicol. 2013 Jun;56:436-42. Doi: 10.1016/J.Fct.2013.02.051. Epub 2013 Mar 14. Anti-Fibrotic Effects Of Puerarin On Ccl4-Induced Hepatic Fibrosis In Rats Possibly Through The Regulation Of Ppar-??...
Liang S, Li Hc, Wang Yx, Wu Ss, Cai Yj, Cui Hl, Yang Yp, Ya J. Dev Growth Differ. 2014 May;56(4):276-92. Doi: 10.1111/Dgd.12129. Epub 2014 Apr 3. Pulmonary Endoderm, Second Heart Field And The Morphogenesis Of Distal Outflow Tract In Mouse Embryon...
Gao S, Zheng Y, Cai Q, Yao W, Wang J, Zhang P, Wang X. J Mater Sci Mater Med. 2014 May;25(5):1283-91. Doi: 10.1007/S10856-014-5150-3. Epub 2014 Jan 23. Comparison Of Morphology And Biocompatibility Of Acellular Nerve Scaffolds Processed By Differe...
Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.
Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...
Chen W, Mo W, Sun K, Huang X, Zhang Yl, Song Hy. Curr Eye Res. 2009 Dec;34(12):1057-64. Doi: 10.3109/02713680903308487. Microplasmin Degrades Fibronectin And Laminin At Vitreoretinal Interface And Outer Retina During Enzymatic Vitrectomy.
Chen Tl, Wang Hl, Liu Yh, Fang Y, Tan Rz, Zhou Ph, Zhou Q, Lv Xy. Scientificworldjournal. 2014;2014:682189. Doi: 10.1155/2014/682189. Epub 2014 Jul 14. Highly Effective Ex Vivo Gene Manipulation To Study Kidney Development Using Self-Complementary...
Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.
He S, Xia T, Wang H, Wei L, Luo X, Li X. Acta Biomater. 2012 Jul;8(7):2659-69. Doi: 10.1016/J.Actbio.2012.03.044. Epub 2012 Apr 3. Multiple Release Of Polyplexes Of Plasmids Vegf And Bfgf From Electrospun Fibrous Scaffolds Towards Regeneration Of ...
Liu Y, Lu J, Li H, Wei J, Li X. Acta Biomater. 2015 Jan;11:114-25. Doi: 10.1016/J.Actbio.2014.10.004. Epub 2014 Oct 7. Engineering Blood Vessels Through Micropatterned Co-Culture Of Vascular Endothelial And Smooth Muscle Cells On Bilayered Electro...
Chen F, Wan H, Xia T, Guo X, Wang H, Liu Y, Li X. Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):699-710. Doi: 10.1016/J.Ejpb.2013.07.009. Epub 2013 Jul 24. Promoted Regeneration Of Mature Blood Vessels By Electrospun Fibers With Loaded Multiple Pdna-C...
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.
Xu L, Zheng N, He Q, Li R, Zhang K, Liang T. Phytomedicine. 2013 Oct 15;20(13):1172-9. Doi: 10.1016/J.Phymed.2013.06.008. Epub 2013 Jul 13. Puerarin, Isolated From Pueraria Lobata (Willd.), Protects Against Hepatotoxicity Via Specific Inhibition O...
Lin Cy, Li Lt, Su Wt. Mater Sci Eng C Mater Biol Appl. 2014 Sep;42:474-8. Doi: 10.1016/J.Msec.2014.05.063. Epub 2014 Jun 6. Three Dimensional Chitosan Scaffolds Influence The Extra Cellular Matrix Expression In Schwann Cells.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lama antibody, laminin alpha 1 antibody