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SKU:PA1822
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-LAMP1 Antibody
Description Rabbit IgG polyclonal antibody for Lysosome-associated membrane glycoprotein 1(LAMP1) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-LAMP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1822)
Replacement Item This antibody may replace the following items: sc-17768|sc-18821|sc-20011|sc-5570|sc-8098|sc-8099 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1(403-417aa YLVGRKRSHAGYQTI), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LAMP1
Protein Name Lysosome-associated membrane glycoprotein 1(LAMP-1)
Molecular Weight 44882 MW
Protein Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Subcellular Localization Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome. This protein shuttles between lysosomes, endosomes, and the plasma membrane. Colocalizes with OSBPL1A at the late endosome.
Uniprot ID P11279
Alternative Names Lysosome-associated membrane glycoprotein 1;LAMP-1;Lysosome-associated membrane protein 1;CD107 antigen-like family member A;CD107a;LAMP1;
Research Areas |neuroscience|cell type marker|neuron marker|soma marker| neuroscience|growth cone| tags & cell markers|subcellular markers|organelles|lysosome| signal transduction|protein trafficking|organelle proteins|neurology process|neurogenesis| stem cells|hematopoietic progenitors|lymphoid|b lymphocytic lineage|t lymphocytic lineage|myeloid|monocytic lineage| cancer|autophagy| metabolism|pathways and processes|metabolism processes|autophagy and mitophagy|cell death|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Lysosome-associated membrane glycoprotein 1(LAMP-1)

LAMP1(lysosomal-associated membrane protein 1) also called LAMPA, LGP120 or CD107A, is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells. By means of in situ hybridization, Mattei et al.(1990) assigned the LAMP1 gene to chromosome 13q34. A related gene, which may be a pseudogene, mapped to chromosome 12p13.3. Hybridization of LAMP1 cDNA to chromosome 12p13.3 was observed even when probes representing different portions of the LAMP1 cDNA were used.

Anti-LAMP1 Antibody Images

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Anti-LAMP1 Antibody
Anti- LAMP1antibody, PA1822, Western blotting
All lanes: Anti LAMP1 (PA1822) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 80KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Do I have to kill the cells before running my WB assay with this anti-LAMP1 antibody?
A: No. The WB assay with this antibody is designed to detect live cells.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lamp1 antibody, lamp 1 antibody, lamp-1 antibody, cd107a antibody