|Product Name||Anti-LAMP2a Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-LAMP2a Rabbit Monoclonal Antibody tested for IP, IHC-P, WB in Human, Mouse, Rat|
|Cite This Product||Anti-LAMP2a Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01573)|
|Immunogen||A synthesized peptide derived from human LAMP2a|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Images And Assay Conditions
Immunohistochemical analysis of paraffin-embedded human liver cancer, using LAMP2a Antibody(M01573)
LAMP2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LAMP2 Antibody (M01573)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of LAMP2a expression in JAR cell lysate (M01573).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 monoclonal antibody (Catalog # M01573) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP2
Protein Target Info (Source: Uniprot.org)
|Protein Name||Lysosome-associated membrane glycoprotein 2|
|Tissue Specificity||Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.|
|Alternative Names||Lysosome-associated membrane glycoprotein 2;LAMP-2;Lysosome-associated membrane protein 2;CD107 antigen-like family member B;Lysosomal membrane glycoprotein type B;LGP-B;CD107b;Lamp2;Lamp-2;|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. This protein shuttles between lysosomes, endosomes, and the plasma membrane.|
|Molecular Weight||45681 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.|
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