|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-LASP1 Antibody|
|Description||Rabbit IgG polyclonal antibody for LIM and SH3 domain protein 1(LASP1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-LASP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1791)|
|Replacement Item||This antibody may replace the following items: sc-103594|sc-103595|sc-134379|sc-292342|sc-374059|sc-398990|sc-8082 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1(16-30aa EKVNCLDKFWHKACF), identical to the related rat sequence and different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||LIM and SH3 domain protein 1(LASP-1)|
|Molecular Weight||29717 MW|
|Protein Function||Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity). .|
|Sequence Similarities||Contains 1 LIM zinc-binding domain.|
|Subcellular Localization||Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Associated with the F- actin rich cortical cytoskeleton. .|
|Alternative Names||LIM and SH3 domain protein 1;LASP-1;Metastatic lymph node gene 50 protein;MLN 50;LASP1;MLN50;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins||
Background for LIM and SH3 domain protein 1(LASP-1)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-LASP1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-LASP1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: A431 Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: 293T Cell Lysate
IHC(P): Human Lung Cancer Tissue
ICC: A549 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,