Anti-LCAT Antibody

LCAT antibody

Boster Bio Anti-LCAT Antibody catalog # PA1967. Tested in WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PA1967
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

Product Name

Anti-LCAT Antibody

View all LCAT Antibodies

SKU/Catalog Number

PA1967

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LCAT Antibody catalog # PA1967. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LCAT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1967)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT, different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1967 is reactive to LCAT in Human, Rat

Applications

PA1967 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

49.578kDa

Background of LCAT

LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed "reverse cholesterol transport" The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For LCAT (Source: Uniprot.org, NCBI)

Gene Name

LCAT

Full Name

Phosphatidylcholine-sterol acyltransferase

Weight

49.578kDa

Superfamily

AB hydrolase superfamily

Alternative Names

EC 2.3.1.43; lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase LCAT lecithin-cholesterol acyltransferase phosphatidylcholine-sterol acyltransferase|1-alkyl-2-acetylglycerophosphocholine esterase|PAF acetylhydrolase|phosphatidylcholine--sterol O-acyltransferase|phospholipid-cholesterol acyltransferase|platelet-activating factor acetylhydrolase|testicular secretory protein Li 24

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LCAT, check out the LCAT Infographic

LCAT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LCAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-LCAT Antibody

Question

I was wanting to use your anti-LCAT antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

As indicated on the product datasheet, PA1967 anti-LCAT antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-13

Question

Will anti-LCAT antibody PA1967 work for WB with brain?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

According to the expression profile of brain, LCAT is highly expressed in brain. So, it is likely that anti-LCAT antibody PA1967 will work for WB with brain.

Boster Scientific Support

Answered: 2020-01-08

Question

My lab would like to test anti-LCAT antibody PA1967 on rat brain for research purposes, then I may be interested in using anti-LCAT antibody PA1967 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

The products we sell, including anti-LCAT antibody PA1967, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-26

Question

Do you have a BSA free version of anti-LCAT antibody PA1967 available?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LCAT antibody PA1967 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-23

Question

Is a blocking peptide available for product anti-LCAT antibody (PA1967)?

Verified Customer

Verified customer

Asked: 2018-05-21

Answer

We do provide the blocking peptide for product anti-LCAT antibody (PA1967). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-21

Question

Would anti-LCAT antibody PA1967 work on zebrafish WB with plasma?

M. Brown

Verified customer

Asked: 2017-07-12

Answer

Our lab technicians have not tested anti-LCAT antibody PA1967 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-LCAT antibody PA1967 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish plasma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-12

Question

Will PA1967 anti-LCAT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Parker

Verified customer

Asked: 2015-07-28

Answer

It shows on the product datasheet, PA1967 anti-LCAT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-07-28

Order DetailsPrice
PA1967

100μg

$370
PA1967-10ug

10μg sample (liquid)

$99
PA1967-Biotin

100 μg Biotin conjugated

$570
PA1967-Cy3

100 μg Cy3 conjugated

$570
PA1967-Dylight488

100 μg Dylight488 conjugated

$570
PA1967-Dylight550

100 μg Dylight550 conjugated

$570
PA1967-Dylight594

100 μg Dylight594 conjugated

$570
PA1967-FITC

100 μg FITC conjugated

$570
PA1967-HRP

100 μg HRP conjugated

$570
PA1967-APC

100 μg APC conjugated

$670
PA1967-PE

100 μg PE conjugated

$670
PA1967-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1967
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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