Anti-Lck Antibody Picoband™

LCK antibody

Boster Bio Anti-Lck Antibody Picoband™ catalog # PB9808. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9808
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-Lck Antibody Picoband™

View all LCK Antibodies

SKU/Catalog Number

PB9808

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lck Antibody Picoband™ catalog # PB9808. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lck Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9808)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lck, different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9808 is reactive to LCK in Human, Mouse, Rat

Applications

PB9808 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

58.001kDa

Background of LCK

Lck (or lymphocyte-specific protein tyrosine kinase) is a 56 kDa protein that is found inside specializedcells of the immune system called lymphocytes. The human LCK gene is mapped to chromosome 1p35-p32. This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For LCK (Source: Uniprot.org, NCBI)

Gene Name

LCK

Full Name

Tyrosine-protein kinase Lck

Weight

58.001kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.10; EC 2.7.10.2; Hck-3; Lck; Lcktkr; Leukocyte C-terminal Src kinase; LSK; LSTRA; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16 LCK IMD22, LSK, YT16, p56lck, pp58lck LCK proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Lck|T-lymphocyte specific protein tyrosine kinase p56lck|leukocyte C-terminal Src kinase|lymphocyte cell-specific protein-tyrosine kinase|p56(LSTRA) protein-tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK|t cell-specific protein-tyrosine kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LCK, check out the LCK Infographic

LCK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LCK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Lck Antibody Picoband™

Question

We have been able to see staining in mouse leukemic t-cell. Do you have any suggestions? Is anti-Lck antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

From what I have seen in literature leukemic t-cell does express LCK. From what I have seen in Uniprot.org, LCK is expressed in thymus, leukemia, leukemic t-cell, lymph, peripheral blood lymphocyte, mammary cancer, cervix carcinoma, among other tissues. Regarding which tissues have LCK expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemia, Pubmed ID: 8139546
Leukemic T-cell, Pubmed ID: 7495859, 19690332
Lymph, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 11840567, 14610046
Peripheral blood lymphocyte, Pubmed ID: 11009097, 2835736, 3489486

Boster Scientific Support

Answered: 2020-04-03

Question

I was wanting to use using your anti-Lck antibody for positive regulation of protein kinase b signaling studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

I appreciate your inquiry. This PB9808 anti-Lck antibody is validated on human tonsil tissue, jurkat whole cell lysate, rat thymus tissue, tissue lysate, spleen tissue, mouse thymus tissue, lymphaden tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-15

Question

We are currently using anti-Lck antibody PB9808 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

The anti-Lck antibody (PB9808) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-01

Question

We ordered your anti-Lck antibody for IHC on cervix carcinoma a few years ago. I am using rat, and I plan to use the antibody for WB next. My lab would like examining cervix carcinoma as well as peripheral blood lymphocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-09-14

Answer

I looked at the website and datasheets of our anti-Lck antibody and it seems that PB9808 has been tested on rat in both IHC and WB. Thus PB9808 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-09-14

Question

My team were content with the WB result of your anti-Lck antibody. However we have observed positive staining in mammary cancer cytoplasm. using this antibody. Is that expected? Could you tell me where is LCK supposed to be expressed?

F. Bhatt

Verified customer

Asked: 2017-03-08

Answer

From literature, mammary cancer does express LCK. Generally LCK expresses in cytoplasm. Regarding which tissues have LCK expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemia, Pubmed ID: 8139546
Leukemic T-cell, Pubmed ID: 7495859, 19690332
Lymph, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 11840567, 14610046
Peripheral blood lymphocyte, Pubmed ID: 11009097, 2835736, 3489486

Boster Scientific Support

Answered: 2017-03-08

Order DetailsPrice
PB9808

100μg

$370
PB9808-10ug

10μg sample (liquid)

$99
PB9808-Biotin

100 μg Biotin conjugated

$570
PB9808-Cy3

100 μg Cy3 conjugated

$570
PB9808-Dylight488

100 μg Dylight488 conjugated

$570
PB9808-Dylight550

100 μg Dylight550 conjugated

$570
PB9808-Dylight594

100 μg Dylight594 conjugated

$570
PB9808-FITC

100 μg FITC conjugated

$570
PB9808-HRP

100 μg HRP conjugated

$570
PB9808-APC

100 μg APC conjugated

$670
PB9808-PE

100 μg PE conjugated

$670
PB9808-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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