Anti-LEKTI Antibody

SPINK5 antibody

Boster Bio Anti-LEKTI Antibody catalog # A03254. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A03254
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-LEKTI Antibody

View all SPINK5 Antibodies

SKU/Catalog Number

A03254

Size

100μl

Form

Liquid

Description

Boster Bio Anti-LEKTI Antibody catalog # A03254. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LEKTI Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03254)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1B1C4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human LEKTI. at AA rangle: 470-550

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03254 is reactive to SPINK5 in Human

Applications

A03254 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

120.714kDa

Background of SPINK5

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For SPINK5 (Source: Uniprot.org, NCBI)

Gene Name

SPINK5

Full Name

Serine protease inhibitor Kazal-type 5

Weight

120.714kDa

Alternative Names

Kazal type 5; LEKTI; LETKI; NETS; serine peptidase inhibitor, Kazal type 5; SPINK5; VAKTI SPINK5 LEKTI, LETKI, NETS, NS, VAKTI serine peptidase inhibitor Kazal type 5 serine protease inhibitor Kazal-type 5|lympho-epithelial Kazal-type-related inhibitor|lymphoepithelial Kazal-type-related inhibitor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SPINK5, check out the SPINK5 Infographic

SPINK5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPINK5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-LEKTI Antibody

Question

Will A03254 anti-LEKTI antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

You can see on the product datasheet, A03254 anti-LEKTI antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-03

Question

Is this A03254 anti-LEKTI antibody reactive to the isotypes of SPINK5?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

The immunogen of A03254 anti-LEKTI antibody is Synthesized peptide derived from the Internal region of human LEKTI. at AA rangle: 470-550. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-12

Question

We are currently using anti-LEKTI antibody A03254 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

The anti-LEKTI antibody (A03254) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-02

Question

Is a blocking peptide available for product anti-LEKTI antibody (A03254)?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

We do provide the blocking peptide for product anti-LEKTI antibody (A03254). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium using anti-LEKTI antibody A03254. Let me know if you need anything else.

P. Singh

Verified customer

Asked: 2016-04-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-04-29

Question

Is there a BSA free version of anti-LEKTI antibody A03254 available?

R. Anderson

Verified customer

Asked: 2014-10-28

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LEKTI antibody A03254 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-10-28

Order DetailsPrice
A03254

100uL

$399

Free Secondary Antibody

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A03254
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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