Anti-LGR4/Gpr48 Monoclonal Antibody

Lgr4/GPR48 antibody

Boster Bio Anti-LGR4/Gpr48 Monoclonal Antibody (Catalog # M02134). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M02134
Size: 100ug
Reactive Species: Human
Host: Mouse
Application: ELISA, WB

Product Name

Anti-LGR4/Gpr48 Monoclonal Antibody

View all Lgr4/GPR48 Antibodies

SKU/Catalog Number

M02134

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-LGR4/Gpr48 Monoclonal Antibody (Catalog # M02134). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-LGR4/Gpr48 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02134)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 6G8.B3.G5.C3

Isotype

IgG2b kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M02134 is reactive to LGR4 in Human

Applications

M02134 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

104.475kDa

Background of Lgr4/GPR48

LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 5 µg/mL
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For LGR4 (Source: Uniprot.org, NCBI)

Gene Name

LGR4

Full Name

Leucine-rich repeat-containing G-protein coupled receptor 4

Weight

104.475kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

GPR48; GPR48G protein-coupled receptor 48; G-protein coupled receptor 48; leucine-rich repeat containing G protein-coupled receptor 4; Lgr4 LGR4 BNMD17, GPR48 leucine rich repeat containing G protein-coupled receptor 4 leucine-rich repeat-containing G-protein coupled receptor 4|G protein-coupled receptor 48

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LGR4, check out the LGR4 Infographic

LGR4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LGR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-LGR4/Gpr48 Monoclonal Antibody

Question

Please see the WB image, lot number and protocol we used for brain using anti-LGR4/Gpr48 Monoclonal antibody M02134. Please let me know if you require anything else.

J. Parker

Verified customer

Asked: 2020-04-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-28

Question

Do you have a BSA free version of anti-LGR4/Gpr48 Monoclonal antibody M02134 available?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LGR4/Gpr48 Monoclonal antibody M02134 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-25

Question

We are currently using anti-LGR4/Gpr48 Monoclonal antibody M02134 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-01

Answer

The anti-LGR4/Gpr48 Monoclonal antibody (M02134) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-01

Question

Is a blocking peptide available for product anti-LGR4/Gpr48 Monoclonal antibody (M02134)?

B. Roberts

Verified customer

Asked: 2018-03-02

Answer

We do provide the blocking peptide for product anti-LGR4/Gpr48 Monoclonal antibody (M02134). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-03-02

Question

Our lab want to know about to test anti-LGR4/Gpr48 Monoclonal antibody M02134 on human brain for research purposes, then I may be interested in using anti-LGR4/Gpr48 Monoclonal antibody M02134 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

The products we sell, including anti-LGR4/Gpr48 Monoclonal antibody M02134, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-26

Question

I see that the anti-LGR4/Gpr48 Monoclonal antibody M02134 works with IHC, what is the protocol used to produce the result images on the product page?

S. Brown

Verified customer

Asked: 2014-06-17

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-06-17

Order DetailsPrice
M02134-100uL

100uL

$612

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$612.00

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