Anti-Leukemia inhibitory factor LIF Antibody

LIF antibody

Boster Bio Anti-Leukemia inhibitory factor LIF Antibody catalog # PA1562. Tested in WB applications. This antibody reacts with Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: PA1562
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Leukemia inhibitory factor LIF Antibody

View all LIF Antibodies

SKU/Catalog Number

PA1562

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Leukemia inhibitory factor LIF Antibody catalog # PA1562. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Leukemia inhibitory factor LIF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1562)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1562 is reactive to LIF in Mouse, Rat

Applications

PA1562 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

22.008kDa

Background of LIF

LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For LIF (Source: Uniprot.org, NCBI)

Gene Name

LIF

Full Name

Leukemia inhibitory factor

Weight

22.008kDa

Superfamily

LIF/OSM family

Alternative Names

CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI LIF CDF, DIA, HILDA, MLPLI LIF interleukin 6 family cytokine leukemia inhibitory factor|D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation-inducing factor|differentiation-stimulating factor|hepatocyte-stimulating factor III|human interleukin in DA cells|melanoma-derived LPL inhibitor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LIF, check out the LIF Infographic

LIF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LIF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1562 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice

Xu B, Sun X, Li L, Wu L, Zhang A, Feng Y. Fertil Steril. 2012 Aug;98(2):389-95. Doi: 10.1016/J.Fertnstert.2012.04.032. Epub 2012 Jun 13. Pinopodes, Leukemia Inhibitory Factor, Integrin-??3, And Mucin-1 Expression In The Peri-Implantation Endometri...

Pei K, Yu C, Shi X, Jia M. Contraception. 2010 Oct;82(4):379-84. Doi: 10.1016/J.Contraception.2010.04.009. Epub 2010 May 23. The Effects Of Mifepristone On The Expressions Of Osteopontin, Interleukin-6 And Leukemia Inhibitory Factor In The Villi O...

Li L, Xu Bf, Chen Qj, Sun Xx. Eur J Obstet Gynecol Reprod Biol. 2010 Aug;151(2):171-5. Doi: 10.1016/J.Ejogrb.2010.04.024. Epub 2010 Jun 9. Effects Of Hydrosalpinx On Pinopodes, Leukaemia Inhibitory Factor, Integrin Beta3 And Muc1 Expression In The...

Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.

Gui J, Xiong F, Yang W, Li J, Huang G. Am J Reprod Immunol. 2012 May;67(5):383-90. Doi: 10.1111/J.1600-0897.2011.01097.X. Epub 2012 Jan 9. Effects Of Acupuncture On Lif And Il-12 In Rats Of Implantation Failure.

Have you used Anti-Leukemia inhibitory factor LIF Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Leukemia inhibitory factor LIF Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-Leukemia inhibitory factor LIF Antibody

Question

My boss were well pleased with the WB result of your anti-LIF antibody. However we have seen positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is LIF supposed to be expressed?

K. Miller

Verified customer

Asked: 2019-10-31

Answer

From literature, left coronary artery does express LIF. Generally LIF expresses in secreted. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197

Boster Scientific Support

Answered: 2019-10-31

Question

We have seen staining in rat embryonic stem cell. What should we do? Is anti-LIF antibody supposed to stain embryonic stem cell positively?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

From what I have seen in literature embryonic stem cell does express LIF. From what I have seen in Uniprot.org, LIF is expressed in left coronary artery, embryonic stem cell, colon, among other tissues. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197

Boster Scientific Support

Answered: 2019-09-27

Question

We are currently using anti-LIF antibody PA1562 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

The anti-LIF antibody (PA1562) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-25

Question

I am looking for using your anti-LIF antibody for positive regulation of astrocyte differentiation studies. Has this antibody been tested with western blotting on intestine tissue? We would like to see some validation images before ordering.

Z. Kulkarni

Verified customer

Asked: 2013-10-16

Answer

We appreciate your inquiry. This PA1562 anti-LIF antibody is tested on rat thymus tissue, intestine tissue, tissue lysate, brain tissue, spleen tissue, mouse liver tissue, kidney tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-10-16

Order DetailsPrice
PA1562

100μg

$370
PA1562-10ug

10μg sample (liquid)

$99
PA1562-Biotin

100 μg Biotin conjugated

$570
PA1562-Cy3

100 μg Cy3 conjugated

$570
PA1562-Dylight488

100 μg Dylight488 conjugated

$570
PA1562-Dylight550

100 μg Dylight550 conjugated

$570
PA1562-Dylight594

100 μg Dylight594 conjugated

$570
PA1562-FITC

100 μg FITC conjugated

$570
PA1562-HRP

100 μg HRP conjugated

$570
PA1562-APC

100 μg APC conjugated

$670
PA1562-PE

100 μg PE conjugated

$670
PA1562-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PA1562
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.