Product Info Summary
SKU: | PA1562 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Leukemia inhibitory factor LIF Antibody
SKU/Catalog Number
PA1562
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leukemia inhibitory factor LIF Antibody catalog # PA1562. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leukemia inhibitory factor LIF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1562)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF, identical to the related rat sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1562 is reactive to LIF in Mouse, Rat
Applications
PA1562 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
22.008kDa
Background of LIF
LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-LIF antibody, PA1562, Western blotting
All lanes: Anti LIF (PA1562) at 0.5ug/ml
Lane 1: Rat Intestine Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Thymus Tissue Lysate at 50ug
Lane 5: Mouse Brain Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 40KD
Protein Target Info & Infographic
Gene/Protein Information For LIF (Source: Uniprot.org, NCBI)
Gene Name
LIF
Full Name
Leukemia inhibitory factor
Weight
22.008kDa
Superfamily
LIF/OSM family
Alternative Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI LIF CDF, DIA, HILDA, MLPLI LIF interleukin 6 family cytokine leukemia inhibitory factor|D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation-inducing factor|differentiation-stimulating factor|hepatocyte-stimulating factor III|human interleukin in DA cells|melanoma-derived LPL inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LIF, check out the LIF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LIF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leukemia inhibitory factor LIF Antibody (PA1562)
Hello CJ!
PA1562 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice
Xu B, Sun X, Li L, Wu L, Zhang A, Feng Y. Fertil Steril. 2012 Aug;98(2):389-95. Doi: 10.1016/J.Fertnstert.2012.04.032. Epub 2012 Jun 13. Pinopodes, Leukemia Inhibitory Factor, Integrin-??3, And Mucin-1 Expression In The Peri-Implantation Endometri...
Pei K, Yu C, Shi X, Jia M. Contraception. 2010 Oct;82(4):379-84. Doi: 10.1016/J.Contraception.2010.04.009. Epub 2010 May 23. The Effects Of Mifepristone On The Expressions Of Osteopontin, Interleukin-6 And Leukemia Inhibitory Factor In The Villi O...
Li L, Xu Bf, Chen Qj, Sun Xx. Eur J Obstet Gynecol Reprod Biol. 2010 Aug;151(2):171-5. Doi: 10.1016/J.Ejogrb.2010.04.024. Epub 2010 Jun 9. Effects Of Hydrosalpinx On Pinopodes, Leukaemia Inhibitory Factor, Integrin Beta3 And Muc1 Expression In The...
Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.
Gui J, Xiong F, Yang W, Li J, Huang G. Am J Reprod Immunol. 2012 May;67(5):383-90. Doi: 10.1111/J.1600-0897.2011.01097.X. Epub 2012 Jan 9. Effects Of Acupuncture On Lif And Il-12 In Rats Of Implantation Failure.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Leukemia inhibitory factor LIF Antibody
Question
My boss were well pleased with the WB result of your anti-LIF antibody. However we have seen positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is LIF supposed to be expressed?
K. Miller
Verified customer
Asked: 2019-10-31
Answer
From literature, left coronary artery does express LIF. Generally LIF expresses in secreted. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-10-31
Question
We have seen staining in rat embryonic stem cell. What should we do? Is anti-LIF antibody supposed to stain embryonic stem cell positively?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
From what I have seen in literature embryonic stem cell does express LIF. From what I have seen in Uniprot.org, LIF is expressed in left coronary artery, embryonic stem cell, colon, among other tissues. Regarding which tissues have LIF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Embryonic stem cell, Pubmed ID: 15146197
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-LIF antibody PA1562 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
The anti-LIF antibody (PA1562) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-25
Question
I am looking for using your anti-LIF antibody for positive regulation of astrocyte differentiation studies. Has this antibody been tested with western blotting on intestine tissue? We would like to see some validation images before ordering.
Z. Kulkarni
Verified customer
Asked: 2013-10-16
Answer
We appreciate your inquiry. This PA1562 anti-LIF antibody is tested on rat thymus tissue, intestine tissue, tissue lysate, brain tissue, spleen tissue, mouse liver tissue, kidney tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-10-16