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SKU:PB9036
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-LIF Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Leukemia inhibitory factor(LIF) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-LIF Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9036)
Replacement Item This antibody may replace the following items: sc-1336|sc-48577|sc-48576|sc-20087|sc-48575 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LIF
Protein Name Leukemia inhibitory factor
Molecular Weight 22008 MW
Protein Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Sequence Similarities Belongs to the LIF/OSM family.
Subcellular Localization Secreted.
Uniprot ID P15018
Alternative Names Leukemia inhibitory factor;LIF;Differentiation-stimulating factor;D factor;Melanoma-derived LPL inhibitor;MLPLI;Emfilermin;LIF;HILDA;
Research Areas |immunology|innate immunity|cytokines|il6 related| immunology| cell biology|cell cycle|cell differentiation| neuroscience|neurology process|neurogenesis| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Leukemia inhibitory factor

LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Anti-LIF Picoband™ Antibody Images

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Anti-LIF Picoband™ Antibody
Anti-LIF Picoband antibody, PB9036-1.JPG
IHC(P): Human Lung Cancer Tissue
Anti-LIF Picoband™ Antibody
Anti-LIF Picoband antibody, PB9036-2.JPG
IHC(P): Human Placenta Tissue
Anti-LIF Picoband™ Antibody
Anti-LIF Picoband antibody, PB9036-3.jpg
All lanes: Anti-LIF(PB9036) at 0.5ug/ml
WB: Recombinant Human LIF Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
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Publications

Xu B, Sun X, Li L, Wu L, Zhang A, Feng Y. Fertil Steril. 2012 Aug;98(2):389-95. Doi: 10.1016/J.Fertnstert.2012.04.032. Epub 2012 Jun 13. Pinopodes, Leukemia Inhibitory Factor, Integrin-??3, And Mucin-1 Expression In The Peri-Implantation Endometriu...
Pei K, Yu C, Shi X, Jia M. Contraception. 2010 Oct;82(4):379-84. Doi: 10.1016/J.Contraception.2010.04.009. Epub 2010 May 23. The Effects Of Mifepristone On The Expressions Of Osteopontin, Interleukin-6 And Leukemia Inhibitory Factor In The Villi O...
Li L, Xu Bf, Chen Qj, Sun Xx. Eur J Obstet Gynecol Reprod Biol. 2010 Aug;151(2):171-5. Doi: 10.1016/J.Ejogrb.2010.04.024. Epub 2010 Jun 9. Effects Of Hydrosalpinx On Pinopodes, Leukaemia Inhibitory Factor, Integrin Beta3 And Muc1 Expression In The...
Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.
Gui J, Xiong F, Yang W, Li J, Huang G. Am J Reprod Immunol. 2012 May;67(5):383-90. Doi: 10.1111/J.1600-0897.2011.01097.X. Epub 2012 Jan 9. Effects Of Acupuncture On Lif And Il-12 In Rats Of Implantation Failure.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.