Anti-LIME1 Purified Monoclonal Antibody

LIME antibody

Boster Bio Anti-LIME1 Purified Monoclonal Antibody (Catalog# M13360). Tested in WB, IHC-P application(s). This antibody reacts with Human.

Product Info Summary

SKU: M13360
Size: 0.1 mg
Reactive Species: Human
Host: Mouse
Application: IHC-P, WB

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Product Name

Anti-LIME1 Purified Monoclonal Antibody

View all LIME Antibodies

SKU/Catalog Number

M13360

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LIME1 Purified Monoclonal Antibody (Catalog# M13360). Tested in WB, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-LIME1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13360)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

LIME-10

Isotype

Mouse IgG2a

Immunogen

COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin. The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M13360 is reactive to LIME1 in Human

Applications

M13360 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

31.288kDa

Background of LIME

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.
Western blotting: Reducing conditions; recommended dilution: 1 μg/ml.

Validation Images & Assay Conditions

Gene/Protein Information For LIME1 (Source: Uniprot.org, NCBI)

Gene Name

LIME1

Full Name

Lck-interacting transmembrane adapter 1

Weight

31.288kDa

Alternative Names

dJ583P15.4; FLJ20406; Lck interacting transmembrane adaptor 1; Lck-interacting membrane protein; Lck-interacting molecule; lck-interacting transmembrane adapter 1; LIMELP8067 LIME1 LIME, dJ583P15.4 Lck interacting transmembrane adaptor 1 lck-interacting transmembrane adapter 1|lck-interacting membrane protein|lck-interacting molecule

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LIME1, check out the LIME1 Infographic

LIME1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LIME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M13360

0.1 mg

$326

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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