Data & Images
|Product Name||Anti-Lipocalin-2/NGAL Antibody|
|Description||Rabbit IgG polyclonal antibody for Neutrophil gelatinase-associated lipocalin(LCN2) detection. Tested with WB, IHC-P in Rat.|
|Cite This Product||Anti-Lipocalin-2/NGAL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1381)|
|Replacement Item||This antibody may replace the following items: sc-22456|sc-50306|sc-22453|sc-50305|sc-48312 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of rat Lipocalin 2(38-53aa LQPGFWTERFQGRWFV).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Neutrophil gelatinase-associated lipocalin(NGAL)|
|Molecular Weight||22476 MW|
|Protein Function||Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth (By similarity). .|
|Subcellular Localization||Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. .|
|Alternative Names||Neutrophil gelatinase-associated lipocalin;NGAL;Alpha-2-microglobulin-related protein;Alpha-2U globulin-related protein;Lipocalin-2;Siderocalin LCN2;p25;Lcn2;|
Background for Neutrophil gelatinase-associated lipocalin(NGAL)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Lipocalin-2/NGAL Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Lipocalin-2/NGAL Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Rat NGAL Protein 10ng
Lane 2: Recombinant Rat NGAL Protein 5ng
Lane 3: Recombinant Rat NGAL Protein 2.5ng
IHC(P): Rat Intestine Tissue Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,