|Validated Species:||Bovine, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-LipoProtein Lipase Antibody|
|Description||Rabbit IgG polyclonal antibody for Lipoprotein lipase(LPL) detection. Tested with WB in Human;Mouse;Rat;Bovine.|
|Cite This Product||Anti-LipoProtein Lipase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1304)|
|Replacement Item||This antibody may replace the following items: sc-32382|sc-58780|sc-32885|sc-373759|sc-32383|sc-81780|sc-73646 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoprotein lipase(448-170aa EKVSHLQKGKAPAVFVKCHDKSL), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Lipoprotein lipase(LPL)|
|Molecular Weight||53162 MW|
|Protein Function||The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). .|
|Sequence Similarities||Belongs to the AB hydrolase superfamily. Lipase family.|
|Subcellular Localization||Cell membrane ; Lipid-anchor, GPI-anchor . Secreted . Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity). .|
|Alternative Names||Lipoprotein lipase;LPL;220.127.116.11;LPL;LIPD;|
|Research Areas|||cardiovascular|lipids / lipoproteins|lipid metabolism|lipases| signal transduction| stem cells|mesenchymal stem cells|adipogenesis| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|obesity||
Background for Lipoprotein lipase(LPL)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-LipoProtein Lipase Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Bovine, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-LipoProtein Lipase Antibody Images
Click the images to enlarge.
All lanes: Anti -Lipoprotein lipase (PA1304) at 0.5ug/ml
Lane 1: Milk
Lane 2: M CF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD,70KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,