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SKU:PA1304
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-LipoProtein Lipase Antibody
SKU/Catalog Number PA1304
Description Rabbit IgG polyclonal antibody for Lipoprotein lipase(LPL) detection. Tested with WB in Human;Mouse;Rat;Bovine.
Cite This Product Anti-LipoProtein Lipase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1304)
Replacement Item This antibody may replace the following items: sc-32382|sc-58780|sc-32885|sc-373759|sc-32383|sc-81780|sc-73646 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Bovine, Human, Mouse, Rat
Predicted Species Horse

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoprotein lipase(448-170aa EKVSHLQKGKAPAVFVKCHDKSL), different from the related mouse and rat sequences by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LPL
Protein Name Lipoprotein lipase(LPL)
Molecular Weight 53162 MW
Protein Function The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). .
Sequence Similarities Belongs to the AB hydrolase superfamily. Lipase family.
Subcellular Localization Cell membrane ; Lipid-anchor, GPI-anchor . Secreted . Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity). .
Uniprot ID P06858
Alternative Names Lipoprotein lipase;LPL;3.1.1.34;LPL;LIPD;
Research Areas |cardiovascular|lipids / lipoproteins|lipid metabolism|lipases| signal transduction| stem cells|mesenchymal stem cells|adipogenesis| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|obesity|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Lipoprotein lipase(LPL)

Lipoprotein lipase(LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. Lipoprotein lipase has been difficult to purify, and its protein sequence remained undetermined until it could be deduced from the nucleotide sequence of its cDNA. The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. Analysis of the sequence indicated that human lipoprotein lipase, hepatic lipase, and pancreatic lipase are members of a gene family. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion.

Anti-LipoProtein Lipase Antibody Images

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Anti-LipoProtein Lipase Antibody
Anti-Lipoprotein lipase antibody, PA1304, Western blotting
All lanes: Anti -Lipoprotein lipase (PA1304) at 0.5ug/ml
Lane 1: Milk
Lane 2: M CF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD,70KD
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Publications

Liu Y, Wang Zb, Yin Wd, Li Qk, Cai Mb, Yu J, Li Hg, Zhang C, Zu Xh. Lipids Health Dis. 2011 Jul 16;10:117. Doi: 10.1186/1476-511X-10-117. Preventive Effect Of Ibrolipim On Suppressing Lipid Accumulation And Increasing Lipoprotein Lipase In The Kid...
Yu Ch, Dai Xy, Chen Q, Zang Jn, Deng Ll, Liu Yh, Ying Hz. Carbohydr Polym. 2013 Apr 15;94(1):56-62. Doi: 10.1016/J.Carbpol.2013.01.006. Epub 2013 Jan 11. Hypolipidemic And Antioxidant Activities Of Polysaccharides From Rosae Laevigatae Fructus In ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lipoprotein lipase antibody, lpl antibody